Product Name
Actin (ACTA1), Monoclonal Antibody
Full Product Name
Anti-Actin Rabbit Monoclonal Antibody
Product Synonym Names
Actin, alpha skeletal muscle; Alpha-actin-1; ACTA1; ACTA
Product Gene Name
anti-ACTA1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P68133
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity-chromatography
Immunogen
A synthesized peptide derived from human Actin
Subcellular Localization
Cytoplasm, cytoskeleton.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-ACTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTA1 antibody
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Product Categories/Family for anti-ACTA1 antibody
Tags & Cell Markers; Subcellular Markers; Cytoskeleton; actin; Signal Transduction; Cytoskeleton/ECM; microfilaments; actin etc; troponin; tropomyosin; actin crosslinking; actin Binding Proteins; actin assembly; Cancer; Invasion/Microenvironment; Apoptosi
Applications Tested/Suitable for anti-ACTA1 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-ACTA1 antibody
WB: 1:1000-1:5000
IP: 1:50
Western Blot (WB) of anti-ACTA1 antibody
Western blot analysis of Actin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate (MBS179785).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACTA1
NCBI/Uniprot data below describe general gene information for ACTA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001091.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001100.3
[Other Products]
UniProt Primary Accession #
P68133
[Other Products]
UniProt Secondary Accession #
P02568; P99020; Q5T8M9[Other Products]
UniProt Related Accession #
P68133[Other Products]
Molecular Weight
42051 MW
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1 [Similar Products]
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
[Similar Products]
NCBI Protein Information
actin, alpha skeletal muscle
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Synonym Protein Names
Alpha-actin-1
UniProt Gene Name
ACTA1 [Similar Products]
UniProt Synonym Gene Names
ACTA [Similar Products]
NCBI Summary for ACTA1
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
UniProt Comments for ACTA1
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Research Articles on ACTA1
1. Shorter than normal thin filament length contributes to the impaired force generation in patients with thin filament myopathy, but only in those who harbor specific mutations in NEB or ACTA1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
