Product Name
ADAMTS13, siRNA
Full Product Name
ADAMTS13 siRNA (Human)
Product Synonym Names
C9orf8; A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAM-TS 13; ADAM-TS13; ADAMTS-13; von Willebrand factor-cleaving protease; vWF-CP; vWF-cleaving protease
Product Gene Name
ADAMTS13 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q76LX8
Specificity
ADAMTS13 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADAMTS13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ADAMTS13 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ADAMTS13 sirna
siRNA to inhibit ADAMTS13 expression using RNA interference
Applications Tested/Suitable for ADAMTS13 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ADAMTS13. It may not necessarily be applicable to this product.
NCBI Accession #
NP_620594.1
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NCBI GenBank Nucleotide #
NM_139025.4
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UniProt Primary Accession #
Q76LX8
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UniProt Secondary Accession #
Q6UY16; Q710F6; Q711T8; Q96L37; Q9H0G3; Q9UGQ1[Other Products]
UniProt Related Accession #
Q76LX8[Other Products]
Molecular Weight
39,864 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 1 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif, 13
NCBI Official Symbol
ADAMTS13 [Similar Products]
NCBI Official Synonym Symbols
VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
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NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Synonym Protein Names
von Willebrand factor-cleaving protease; vWF-CP; vWF-cleaving protease
Protein Family
A disintegrin and metalloproteinase with thrombospondin motifs
UniProt Gene Name
ADAMTS13 [Similar Products]
UniProt Synonym Gene Names
C9orf8; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease [Similar Products]
UniProt Entry Name
ATS13_HUMAN
NCBI Summary for ADAMTS13
This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Comments for ADAMTS13
ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.24.87; Calcium-binding; Protease; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Extracellular matrix
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen
Molecular Function: integrin binding; protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding
Biological Process: integrin-mediated signaling pathway; protein amino acid O-linked glycosylation; platelet activation; cellular protein metabolic process; glycoprotein metabolic process; peptide catabolic process; response to toxin; cell-matrix adhesion; protein processing; post-translational protein modification; proteolysis
Disease: Thrombotic Thrombocytopenic Purpura, Congenital
Research Articles on ADAMTS13
1. assay may serve as a valuable research tool to investigate the biochemical characteristics of VWF and ADAMTS13 under shear flow.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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