C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1), ELISA Kit

MCF3; ROS; c-Ros-1; v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c-ros oncogene 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1).
No significant cross-reactivity or interference between C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ROS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2705437 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ROS1. The ELISA analytical biochemical technique of the MBS2705437 kit is based on ROS1 antibody-ROS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

263,915 Da

ROS proto-oncogene 1, receptor tyrosine kinase

proto-oncogene tyrosine-protein kinase ROS

Proto-oncogene tyrosine-protein kinase ROS

MCF3; ROS  [Similar Products]

This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]

ROS: a proto-oncogenic receptor tyrosine kinase whose expression is tightly restricted during development. Normally expressed in ***** murine and human epithelial cells of the epididymis. Transgenic mice lacking the c-ros gene are infertile. Ectopic expression of c-Ros has been reported in meningiomas and astrocytomas. Is up-regulated in human glioma: 30% of malignant glioma tumors are ROS positive. An oncogenic fusion protein between PIST (aka FIG) and ROS, resulting from an intra-chromosomal homozygous deletion of 240 kilobases on 6q21, is found in glioblastoma multiform. PIST (aka FIG) is a peripheral membrane protein associated with the Golgi apparatus. Unlike other fusion RTK oncogenes, the mechanism of activation of PIST-ROS does not appear to be dimerization: the PIST-ROS fusion protein appears to be monomeric in vivo. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region of PIST, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. c-ROS may also be activated epigenetically, suggesting caution when using 5-aza-dC for treating glioma.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); Sev family; TK group

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: cell surface; integral component of membrane; membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; protein binding; protein phosphatase binding; protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell differentiation; cell proliferation; columnar/cuboidal epithelial cell development; negative regulation of gene expression; peptidyl-tyrosine autophosphorylation; protein amino acid phosphorylation; regulation of cell growth; regulation of ERK1 and ERK2 cascade; regulation of phosphate transport; regulation of TOR signaling; spermatogenesis; transmembrane receptor protein tyrosine kinase signaling pathway

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