Product Name
HPRT1, siRNA
Full Product Name
HPRT1 siRNA (Rat)
Product Synonym Names
HPRT; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase
Product Gene Name
HPRT1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P27605
Specificity
HPRT1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HPRT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HPRT1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HPRT1 sirna
siRNA to inhibit HPRT1 expression using RNA interference
Applications Tested/Suitable for HPRT1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HPRT1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036715.1
[Other Products]
NCBI GenBank Nucleotide #
NM_012583.2
[Other Products]
UniProt Primary Accession #
P27605
[Other Products]
UniProt Secondary Accession #
P70469; Q4KMC5; Q62926[Other Products]
UniProt Related Accession #
P27605[Other Products]
Molecular Weight
24,477 Da
NCBI Official Full Name
hypoxanthine-guanine phosphoribosyltransferase
NCBI Official Synonym Full Names
hypoxanthine phosphoribosyltransferase 1
NCBI Official Symbol
Hprt1 [Similar Products]
NCBI Official Synonym Symbols
Hprt; Hgprtase
[Similar Products]
NCBI Protein Information
hypoxanthine-guanine phosphoribosyltransferase
UniProt Protein Name
Hypoxanthine-guanine phosphoribosyltransferase
Protein Family
Hypoxanthine-guanine phosphoribosyltransferase
UniProt Gene Name
Hprt1 [Similar Products]
UniProt Synonym Gene Names
Hprt; HGPRT; HGPRTase [Similar Products]
UniProt Entry Name
HPRT_RAT
NCBI Summary for HPRT1
catalyzes the conversion of IMP and diphosphate to hypoxanthine and 5-phospho-alpha-D-ribose 1-diphosphate [RGD, Feb 2006]
UniProt Comments for HPRT1
HPRT1: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS). LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation. Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT); also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Protein type: Nucleotide Metabolism - purine; Xenobiotic Metabolism - drug metabolism - other enzymes; Cell development/differentiation; Transferase; EC 2.4.2.8
Cellular Component: cytoplasm; cytosol
Molecular Function: identical protein binding; protein homodimerization activity; nucleotide binding; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding
Biological Process: hypoxanthine metabolic process; IMP salvage; cytolysis; adenine salvage; locomotory behavior; dopamine metabolic process; protein homotetramerization; cerebral cortex neuron differentiation; IMP metabolic process; hypoxanthine salvage; adenine metabolic process; central nervous system neuron development; grooming behavior; lymphocyte proliferation; striatum development; GMP salvage; dendrite morphogenesis; response to amphetamine; purine nucleotide biosynthetic process; GMP catabolic process; positive regulation of dopamine metabolic process; cellular response to insulin stimulus; spermatogenesis; purine ribonucleoside salvage; brain development; guanine salvage
Research Articles on HPRT1
1. Thsi study validated the Hprt1, Actb, Mapk6 or B2m and showed that any one or combination of two of them are good reference genes for normalization of mRNA expression in qPCR experiments in the spinal cord and RVM in the CFA model of inflammatory injury.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
