HPRT1, Polyclonal Antibody

HPRT; HGPRT

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

1mg/ml (lot specific)

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-HPRT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Polyclonal

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Western blot analysis of extracts of various cell lines, using HPRT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

218

hypoxanthine phosphoribosyltransferase 1

hypoxanthine-guanine phosphoribosyltransferase; HGPRTase

Hypoxanthine-guanine phosphoribosyltransferase

HPRT; HGPRT; HGPRTase  [Similar Products]

HPRT_HUMAN

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]

HPRT1: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS). LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation. Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT); also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Protein type: EC 2.4.2.8; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Nucleotide Metabolism - purine; Cell development/differentiation

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding; nucleotide binding

Biological Process: grooming behavior; lymphocyte proliferation; hypoxanthine metabolic process; IMP salvage; striatum development; nucleobase, nucleoside and nucleotide metabolic process; GMP salvage; dendrite morphogenesis; cytolysis; response to amphetamine; locomotory behavior; adenine salvage; purine nucleotide biosynthetic process; dopamine metabolic process; purine base metabolic process; purine salvage; cerebral cortex neuron differentiation; protein homotetramerization; positive regulation of dopamine metabolic process; GMP catabolic process; IMP metabolic process; hypoxanthine salvage; purine ribonucleoside salvage; central nervous system neuron development; guanine salvage

Disease: Kelley-seegmiller Syndrome; Lesch-nyhan Syndrome

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