AKR1B1, Polyclonal Antibody

ADR; AKR1B 1; Akr1b1; Aldehyde reductase 1; Aldehyde reductase; Aldose reductase; aldr 1; aldr1; ALR2; AR; P15121; aldo-keto reductase family 1 member B

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Immunogen affinity purified.

Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-AKR1B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit IgG polyclonal antibody for Aldose reductase(AKR1B1) detection.
Background: Aldo-keto reductase family 1, member B1 (aldose reductase), also known as AR, is an enzyme that in humans is encoded by the AKR1B1 gene. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number ofaldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot: 0.1-0.5ug/ml
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml

Western blot analysis of AKR1B1 expression in rat cardiac muscle extract (lane 1), human placenta extract (lane 2) and HELA whole cell lysates (lane 3). AKR1B1 at 36KD was detected using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.

AKR1B1 was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.

AKR1B1 was detected in paraffin-embedded sections of rat adrenal gland tissues using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.

AKR1B1 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.

35,853 Da

aldo-keto reductase family 1 member B

aldose reductase

Aldose reductase

ALDR1; AR  [Similar Products]

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - pyruvate; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: aldehyde reductase activity; aldo-keto reductase activity; electron carrier activity; glyceraldehyde oxidoreductase activity

Biological Process: C21-steroid hormone biosynthetic process; carbohydrate metabolic process; fructose biosynthetic process; response to stress

1. Ferraretto A, Negri A, Giuliani A, et al. (1993). "Aldose reductase is involved in long-term adaptation of EUE cells to hyperosmotic stress".Biochim. Biophys. Acta 1175(3): 283-8.
2. Graham A, Heath P, Morten JE, Markham AF (March 1991). "The human aldose reductase gene maps to chromosome region 7q35". Hum. Genet. 86 (5): 509-14.

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