HPRT1, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Purified by antigen-affinity chromatography.

Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Small volumes of anti-HPRT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200

Sample (30 ug of whole cell lysate) A: IMR3212% SDS PAGE Primary antibody diluted at 1: 1000

Immunohistochemical analysis of paraffin-embedded AdCA, using HPRT antibody at 1: 500 dilution.

Immunofluorescence analysis of methanol-fixed A549, using HPRT antibody at 1: 500 dilution.

24,579 Da

hypoxanthine phosphoribosyltransferase 1

hypoxanthine-guanine phosphoribosyltransferase

Hypoxanthine-guanine phosphoribosyltransferase

HPRT; HGPRT; HGPRTase  [Similar Products]

HPRT_HUMAN

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]

HPRT1: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS). LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation. Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT); also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Protein type: Transferase; Cell development/differentiation; EC 2.4.2.8; Nucleotide Metabolism - purine; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; nucleotide binding; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding

Biological Process: hypoxanthine metabolic process; IMP salvage; cytolysis; adenine salvage; locomotory behavior; dopamine metabolic process; purine salvage; protein homotetramerization; cerebral cortex neuron differentiation; IMP metabolic process; hypoxanthine salvage; central nervous system neuron development; grooming behavior; lymphocyte proliferation; striatum development; GMP salvage; nucleobase, nucleoside and nucleotide metabolic process; dendrite morphogenesis; response to amphetamine; purine nucleotide biosynthetic process; purine base metabolic process; positive regulation of dopamine metabolic process; GMP catabolic process; purine ribonucleoside salvage; guanine salvage

Disease: Kelley-seegmiller Syndrome; Lesch-nyhan Syndrome

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