Product Name
ATP6V1B2, siRNA
Full Product Name
ATP6V1B2 siRNA (Human)
Product Synonym Names
ATP6B2; VPP3; V-type proton ATPase subunit B. brain isoform; V-ATPase subunit B 2; Endomembrane proton pump 58 kDa subunit; HO57; Vacuolar proton pump subunit B 2
Product Gene Name
ATP6V1B2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P21281
Specificity
ATP6V1B2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATP6V1B2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ATP6V1B2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATP6V1B2 sirna
siRNA to inhibit ATP6V1B2 expression using RNA interference
Applications Tested/Suitable for ATP6V1B2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ATP6V1B2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001684.2
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NCBI GenBank Nucleotide #
NM_001693.3
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UniProt Primary Accession #
P21281
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UniProt Secondary Accession #
Q14544; Q15859; Q96IR0; B2R5Z3; D3DSQ5[Other Products]
UniProt Related Accession #
P21281[Other Products]
Molecular Weight
56,501 Da
NCBI Official Full Name
V-type proton ATPase subunit B, brain isoform
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
NCBI Official Symbol
ATP6V1B2 [Similar Products]
NCBI Official Synonym Symbols
HO57; VATB; VPP3; Vma2; ATP6B2; ATP6B1B2
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NCBI Protein Information
V-type proton ATPase subunit B, brain isoform
UniProt Protein Name
V-type proton ATPase subunit B, brain isoform
UniProt Synonym Protein Names
Endomembrane proton pump 58 kDa subunit; HO57; Vacuolar proton pump subunit B 2
Protein Family
V-type proton ATPase
UniProt Gene Name
ATP6V1B2 [Similar Products]
UniProt Synonym Gene Names
ATP6B2; VPP3; V-ATPase subunit B 2 [Similar Products]
UniProt Entry Name
VATB2_HUMAN
NCBI Summary for ATP6V1B2
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]
UniProt Comments for ATP6V1B2
ATP6V1B2: Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the ATPase alpha/beta chains family.
Protein type: Energy Metabolism - oxidative phosphorylation; Hydrolase; EC 3.6.3.14
Chromosomal Location of Human Ortholog: 8p21.3
Cellular Component: ruffle; microvillus; intracellular membrane-bound organelle; lysosomal membrane; melanosome; plasma membrane; integral to membrane; endomembrane system; cytosol
Molecular Function: hydrogen ion transmembrane transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism; ATP binding
Biological Process: ATP metabolic process; interaction with host; proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport
Disease: Zimmermann-laband Syndrome 2
Research Articles on ATP6V1B2
1. A missense mutation in ATP6V1B2 associated with Zimmermann-Laband syndrome.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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