Product Name
PCBD1, siRNA
Full Product Name
PCBD1 siRNA (Mouse)
Product Synonym Names
DCOH; PCBD; Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD
Product Gene Name
PCBD1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P61458
Specificity
PCBD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PCBD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PCBD1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PCBD1 sirna
siRNA to inhibit PCBD1 expression using RNA interference
Applications Tested/Suitable for PCBD1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PCBD1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_079549.1
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NCBI GenBank Nucleotide #
NM_025273.4
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UniProt Primary Accession #
P61458
[Other Products]
UniProt Secondary Accession #
P70519; P80095; Q9D930[Other Products]
UniProt Related Accession #
P61458[Other Products]
Molecular Weight
11,986 Da
NCBI Official Full Name
pterin-4-alpha-carbinolamine dehydratase
NCBI Official Synonym Full Names
pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 1
NCBI Official Symbol
Pcbd1 [Similar Products]
NCBI Official Synonym Symbols
Pcd; Phs; Dcoh; Pcbd
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NCBI Protein Information
pterin-4-alpha-carbinolamine dehydratase
UniProt Protein Name
Pterin-4-alpha-carbinolamine dehydratase
UniProt Synonym Protein Names
4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD
Protein Family
Pterin-4-alpha-carbinolamine dehydratase
UniProt Gene Name
Pcbd1 [Similar Products]
UniProt Synonym Gene Names
Dcoh; Pcbd; PHS; DCoH; Dimerization cofactor of HNF1; PCD [Similar Products]
UniProt Entry Name
PHS_MOUSE
NCBI Summary for PCBD1
This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. [provided by RefSeq, Apr 2015]
UniProt Comments for PCBD1
PCBD1: Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity. Defects in PCBD1 are the cause of BH4-deficient hyperphenylalaninemia type D (HPABH4D); also known as hyperphenylalaninemia with primapterinuria. HPABH4D is characterized by the excretion of 7-substituted pterins in the urine of affected patients. Belongs to the pterin-4-alpha-carbinolamine dehydratase family.
Protein type: Transcription, coactivator/corepressor; Lyase; EC 4.2.1.96; Oxidoreductase
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: identical protein binding; protein binding; 4-alpha-hydroxytetrahydrobiopterin dehydratase activity; transcription coactivator activity; lyase activity; phenylalanine 4-monooxygenase activity
Biological Process: tetrahydrobiopterin biosynthetic process; regulation of transcription, DNA-dependent; transcription, DNA-dependent; protein heterooligomerization; regulation of protein homodimerization activity; positive regulation of transcription, DNA-dependent; protein homotetramerization
Research Articles on PCBD1
1. Mouse Pcbd1 is a moonlighting protein that has dehydratase activity in addition to functioning as a cofactor which regulates HNF1alpha.
Precautions
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Disclaimer
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