Product Name
Neuroligin 3 (NLGN3), Monoclonal Antibody
Full Product Name
Neuroligin 3 Antibody, Clone S110-29: Dylight 350
Product Synonym Names
Mouse Anti-Rat Neuroligin 3 Monoclonal IgG1; Neuroligin 3 Antibody: Dylight 350; Gliotactin homolog Antibody; Neuroligin-3 Antibody; Nlgn3 Antibody; NLGN3_HUMAN Antibody
Product Gene Name
anti-NLGN3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q62889
Species Reactivity
Human, Mouse, Rat
Specificity
Detects ~110 kDa. Does not cross-react with Neuroligin-1,-2,-4 or-4.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.1% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3. Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2.
Cellular Localization
Cell Membrane; Cell Junction; Synapse
Certificate of Analysis
1 ug/ml was sufficient for detection of Neuroligin 3 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-NLGN3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NLGN3 antibody
Neuroligins are Type I membrane proteins enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. They have been characterized as neuronal cell surface proteins and are thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. They play a major role in the formation or maintenance of synaptic junctions. They are also thought to be involved in the specification of excitatory synapses. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses.
Product Categories/Family for anti-NLGN3 antibody
Neuroscience; Cell Markers; Neuron Markers; Postsynaptic Markers
Applications Tested/Suitable for anti-NLGN3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-NLGN3 antibody
WB: 1:1000
Immunocytochemistry (ICC) of anti-NLGN3 antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 3 Antibody. (D) Composite.
Western Blot (WB) of anti-NLGN3 antibody
Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3 protein using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.
NCBI/Uniprot data below describe general gene information for NLGN3. It may not necessarily be applicable to this product.
NCBI Accession #
AAA97871
[Other Products]
UniProt Primary Accession #
Q62889
[Other Products]
UniProt Related Accession #
Q62889[Other Products]
NCBI Official Full Name
neuroligin 3
NCBI Official Synonym Full Names
neuroligin 3
NCBI Official Symbol
Nlgn3 [Similar Products]
NCBI Protein Information
neuroligin-3
UniProt Protein Name
Neuroligin-3
UniProt Synonym Protein Names
Gliotactin homolog
Protein Family
Neuroligin
UniProt Gene Name
Nlgn3 [Similar Products]
NCBI Summary for NLGN3
neuronal cell surface protein that binds neurexins [RGD, Feb 2006]
UniProt Comments for NLGN3
Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system.
Research Articles on NLGN3
1. Results showed that in pyramidal and interneurons, neuroligin 1 and neuroligin 3 were involved in excitatory synapse formation, and neuroligin 2 in GABAergic synapse formation.
Precautions
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Disclaimer
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