Neuroligin 3, Monoclonal Antibody

Gliotactin homolog; Neuroligin-3; Nlgn3; NLGN3_HUMAN

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

S110-29

Mouse

Protein G Purified

1mg/mL (lot specific)

-20 degree C

Small volumes of anti-Nlgn3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background Info: Detects a 110 kDa protein. Does not cross-react with Neuroligin-1, -2, -4 or -4.

Scientific Background: Neuroligins are Type I membrane proteins enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. They have been characterized as neuronal cell surface proteins and are thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. They play a major role in the formation or maintenance of synaptic junctions. They are also thought to be involved in the specification of excitatory synapses. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses.

Neuroscience

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

1:1000 WB

Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3 protein using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29. Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.

91,563 Da[Similar Products]

neuroligin 3

neuroligin-3; gliotactin homolog

Neuroligin-3

NLGN3_RAT

neuronal cell surface protein that binds neurexins [RGD, Feb 2006]

NLGN3: Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and may mediate its effects by clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system. Defects in NLGN3 may be the cause of susceptibility to autism X-linked type 1 (AUTSX1). AUTSX1 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Defects in NLGN3 may be the cause of susceptibility to X- linked Asperger syndrome 1 (ASPGX1). ASPGX1 is considered to be a form of childhood autism. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: extracellular space; cell surface; endocytic vesicle; integral to plasma membrane; excitatory synapse; synapse; cell junction

Molecular Function: neurexin binding; cell adhesion molecule binding; receptor activity

Biological Process: regulation of synaptic transmission; receptor-mediated endocytosis; rhythmic synaptic transmission; regulation of synaptic transmission, glutamatergic; axon extension; metabolic process; neurological control of breathing; learning; social behavior; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; regulation of inhibitory postsynaptic membrane potential; synaptogenesis; ***** behavior; synapse organization and biogenesis; oligodendrocyte differentiation; visual learning; neuron adhesion; cell adhesion; regulation of excitatory postsynaptic membrane potential

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