MYO5A, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity purification

Store at -20 degree C. Avoid freeze / thaw cycles.

Small volumes of anti-MYO5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Polyclonal

Western Blot (WB)

WB: 1:500 - 1:2000

Western blot analysis of extracts of various cell lines, using MYO5A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat kidney using MYO5A antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using MYO5A antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate cancer using MYO5A antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human rectum using MYO5A antibody at dilution of 1:100 (40x lens).

Calculated MW: 215kDa
Molecular Weight: 1855

myosin VA

unconventional myosin-Va

Unconventional myosin-Va

MYH12  [Similar Products]

MYO5A_HUMAN

This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]

MYO5A: Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. May also be required for some polarization process involved in dendrite formation. May be a homodimer, which associates with multiple calmodulin or myosin light chains. Binds MLPH and MYRIP. Interacts with RIPL2, the interaction is required for its role in dendrite formation. Detected in melanocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: actin filament; actomyosin; cell soma; cytoplasm; cytosol; early endosome; endoplasmic reticulum; filopodium tip; Golgi apparatus; growth cone; intermediate filament; late endosome; lysosome; melanosome; membrane; myosin complex; neuron projection; peroxisome; photoreceptor outer segment; recycling endosome; ruffle; vesicle

Molecular Function: actin binding; ATP binding; calcium ion binding; calmodulin binding; microfilament motor activity; Rab GTPase binding

Biological Process: actin filament-based movement; cellular protein metabolic process; cellular response to insulin stimulus; ER localization; exocytosis; insulin secretion; locomotion during locomotory behavior; long-chain fatty acid biosynthetic process; melanin biosynthetic process; melanocyte differentiation; melanosome transport; myelination; odontogenesis; post-Golgi vesicle-mediated transport; regulation of inositol-1,4,5-triphosphate receptor activity; secretory granule localization; synapse organization and biogenesis; synaptic transmission; transport; vesicle transport along actin filament; vesicle-mediated transport; visual perception

Disease: Griscelli Syndrome, Type 1

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