Product Name
Histone H3 (H3F3A), Blocking Peptide
Full Product Name
Histone H3 Blocking Peptide
Product Synonym Names
H3.3A; H3F3; H3.3B; Histone H3.3
Product Gene Name
H3F3A blocking peptide
[Similar Products]
Histone H3 peptide (MBS828930) is used for blocking the activity of Histone H3 antibody (MBS8206852)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P84243
Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Porcine, Zebrafish
Form/Format
Lyophilized powder
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Directions for Use
Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Other Notes
Small volumes of H3F3A blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
H3F3A blocking peptide
The peptide is used to block Anti-Histone H3 Antibody reactivity.
Applications Tested/Suitable for H3F3A blocking peptide
Blocking (BL)
NCBI/Uniprot data below describe general gene information for H3F3A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002098.1
[Other Products]
NCBI GenBank Nucleotide #
NM_002107.4
[Other Products]
UniProt Primary Accession #
P84243
[Other Products]
UniProt Secondary Accession #
P06351; P33155; Q5VV55; Q5VV56; Q66I33; Q9V3W4[Other Products]
UniProt Related Accession #
P84243[Other Products]
Molecular Weight
15,328 Da
NCBI Official Full Name
histone H3.3
NCBI Official Synonym Full Names
H3 histone, family 3A
NCBI Official Symbol
H3F3A [Similar Products]
NCBI Official Synonym Symbols
H3F3; H3.3A
[Similar Products]
NCBI Protein Information
histone H3.3
UniProt Protein Name
Histone H3.3
UniProt Gene Name
H3F3A [Similar Products]
UniProt Synonym Gene Names
H3.3A; H3F3 [Similar Products]
UniProt Entry Name
H33_HUMAN
NCBI Summary for H3F3A
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008]
UniProt Comments for H3F3A
H3F3A: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Belongs to the histone H3 family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 1q42.12
Cellular Component: nucleoplasm; nuclear chromosome; protein complex; extracellular region; nucleosome; nucleus
Molecular Function: protein binding; nucleosomal DNA binding; protein heterodimerization activity
Biological Process: chromatin silencing at rDNA; nucleosome assembly; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; gene expression; blood coagulation; regulation of gene expression, epigenetic; positive regulation of cell growth; DNA methylation on cytosine
Research Articles on H3F3A
1. On the basis of our findings, H3F3A p.Gly34 Trp or p.Gly34 Leu mutations are not a frequent event in CGCL.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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