Product Name
Fli-1 (FLI1), Polyclonal Antibody
Product Synonym Names
Anti -Fli-1
Product Gene Name
anti-FLI1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
chromosome: 11; Location: 11q24.1-q24.3
3D Structure
ModBase 3D Structure for Q01543
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes human Fli-1. Species Crossreactivity: mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Immunogen
Synthetic peptide derived from C-terminus of human Fli-1. Cellular Localization: Nuclear. MW of Antigen: ~50kD.
Positive Control
Jurkat cells
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-FLI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-FLI1 antibody
Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.
Product Categories/Family for anti-FLI1 antibody
Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-FLI1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-FLI1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 2-4ug/ml for 2hrs at RT.
NCBI/Uniprot data below describe general gene information for FLI1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
Q01543
[Other Products]
UniProt Secondary Accession #
Q14319; Q92480; Q9UE07; B2R8H2[Other Products]
UniProt Related Accession #
Q01543; Q9UEE2; Q9UEE3[Other Products]
Molecular Weight
50,982 Da[Similar Products]
NCBI Official Full Name
FLI-1
NCBI Official Synonym Full Names
Friend leukemia virus integration 1
NCBI Official Symbol
FLI1 [Similar Products]
NCBI Official Synonym Symbols
EWSR2; SIC-1; FLI1
[Similar Products]
NCBI Protein Information
Friend leukemia integration 1 transcription factor; proto-oncogene Fli-1; transcription factor ERGB
UniProt Protein Name
Friend leukemia integration 1 transcription factor
UniProt Synonym Protein Names
Proto-oncogene Fli-1; Transcription factor ERGB
Protein Family
Friend leukemia integration 1 transcription factor
UniProt Gene Name
FLI1 [Similar Products]
UniProt Entry Name
FLI1_HUMAN
NCBI Summary for FLI1
This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Comments for FLI1
FLI1: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Defects in FLI1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 11q24.1-q24.3
Cellular Component: nucleus
Molecular Function: DNA binding; chromatin binding; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; organ morphogenesis; hemostasis; blood circulation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation
Research Articles on FLI1
1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
2. report FLI-1 expression in an expanded series of 75 cases of T-cell lymphoma and found high expression in anaplastic large cell lymphoma and angioimmunoblastic T-cell lymphoma
3. EWS-FLI-1 and miRNA-145 function in a mutually repressive feedback loop and identify their common target gene, SOX2, in addition to miRNA145 itself, as key players in Ewing sarcoma family tumors cell differentiation and tumorigenicity
4. Propsectively evaluated the impact of EWS-FLI1/ERG fusions on disease progression in Ewing's sarcoma/peripheral primitive neuroectodermal tumor.
5. Intensive treatment protocols for localized Ewing family sarcoma have erased the clinical differences observed in patients with EWS-FLI1/ERG fusions.
6. EWS/FLI1 inhibits both DKK-1 expression as well as beta-catenin/TCF-dependent transcription, which could contribute to progression of tumours of the Ewing family.
7. EWS/FLI1 inhibits both DKK-1 expression as well as beta-catenin/TCF-dependent transcription, which could contribute to progression of tumours of the Ewing family.
8. Findings suggest that EWS/FLI1 induces apoptosis, at least partially, through the activation of CASP3.
9. GLI1 is a central mediator of EWS/FLI1 signaling in Ewing tumors
10. results suggest that Fli-1 contributes to the malignancy of human breast cancer by inhibiting apoptosis through upregulated expression of the bcl-2 gene.
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