Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against ENPP1. It has been selected for its ability to recognize ENPP1 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of anti-ENPP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Recombinant protein.

DAB staining on IHC-P; Samples: Human Liver cancer Tissue)

104,924 Da

ectonucleotide pyrophosphatase/phosphodiesterase 1

ectonucleotide pyrophosphatase/phosphodiesterase family member 1

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1

M6S1; NPPS; PC1; PDNP1; NPPase  [Similar Products]

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.4.1; EC 3.6.1.9; Membrane protein, integral; Motility/polarity/chemotaxis; Nucleotide Metabolism - purine; Phosphatase (non-protein); Phosphodiesterase

Chromosomal Location of Human Ortholog: 6q23.2

Cellular Component: basolateral plasma membrane; cell surface; extracellular space; integral component of membrane; integral component of plasma membrane; lysosomal membrane; plasma membrane

Molecular Function: 3'-phosphoadenosine 5'-phosphosulfate binding; ATP binding; calcium ion binding; exonuclease activity; insulin receptor binding; nucleic acid binding; nucleoside-triphosphate diphosphatase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; protein binding; protein homodimerization activity; scavenger receptor activity; zinc ion binding

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; ATP metabolic process; biomineral formation; cellular phosphate ion homeostasis; cellular response to insulin stimulus; generation of precursor metabolites and energy; immune response; inorganic diphosphate transport; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of protein autophosphorylation; nucleic acid phosphodiester bond hydrolysis; nucleoside triphosphate catabolic process; phosphate-containing compound metabolic process; receptor-mediated endocytosis; regulation of bone mineralization; riboflavin metabolic process; sequestering of triacylglycerol

Disease: Arterial Calcification, Generalized, Of Infancy, 1; Cole Disease; Diabetes Mellitus, Noninsulin-dependent; Hypophosphatemic Rickets, Autosomal Recessive, 2; Obesity

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