RNH2C, Polyclonal Antibody

Ribonuclease H2 subunit C; RNase H2 subunit C; Aicardi-Goutieres syndrome 3 protein; AGS3; RNase H1 small subunit; Ribonuclease HI subunit C; RNASEH2C; AYP1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This RNH2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-108 amino acids from the Central region of human RNH2C.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-RNASEH2C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

RNH2C encodes a ribonuclease H subunit that can cleave ribonucleotides from RNA:DNA duplexes.

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

WB~~1:1000

Western blot analysis of RNH2C Antibody (Center) in HL-60 cell line lysates (35ug/lane). RNH2C(arrow) was detected using the purified Pab.

RNH2C Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

17840

ribonuclease H2, subunit C

ribonuclease H2 subunit C

Ribonuclease H2 subunit C

AYP1; RNase H2 subunit C; AGS3  [Similar Products]

RNH2C_HUMAN

This gene encodes a ribonuclease H subunit that can cleave ribonucleotides from RNA:DNA duplexes. Mutations in this gene cause Aicardi-Goutieres syndrome-3, a disease that causes severe neurologic dysfunction. A pseudogene for this gene has been identified on chromosome Y, near the sex determining region Y (SRY) gene. [provided by RefSeq, Jul 2008]

RNASEH2C: Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging- strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. Defects in RNASEH2C are the cause of Aicardi-Goutieres syndrome type 3 (AGS3). A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood. Belongs to the RNase H2 subunit C family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: nucleus

Biological Process: RNA catabolic process

Disease: Aicardi-goutieres Syndrome 3

Chon,H., et.al., Nucleic Acids Res. 37 (1), 96-110 (2009)
Rice,G., et.al., Am. J. Hum. Genet. 81 (4), 713-725 (2007)
Crow,Y.J., et.al., Nat. Genet. 38 (8), 910-916 (2006)

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