AHCY, Recombinant Protein

Adenosylhomocysteinase isoform 1; AHCY; adoHcyase; SAHH

For Research Use Only. Not for use in diagnostic procedures.

Baculovirus

> 90% by SDS-PAGE

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.

0.25mg/ml (determined by Absorbance at 280nm) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of AHCY recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

AHCY, also known as adenosylhomocysteinase isoform 1, is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCYs activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease. It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adenosyl-L-methionine methyltransferases. Recombinant human AHCY protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Signal Transduction; Metabolism

SDS-PAGE

48.8kDa (441aa) 40-57KDa (SDS-PAGE under reducing conditions.)

adenosylhomocysteinase

adenosylhomocysteinase

Adenosylhomocysteinase

SAHH; AdoHcyase  [Similar Products]

S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.

Vugrek O. et al., (2009) Hum Mutat. 30: E555-65.; Park SJ. et al., (2015) Am J Cancer Res. 5: 2127-2138.

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