Product Name
CCDC88B, siRNA
Full Product Name
CCDC88B siRNA (Human)
Product Synonym Names
BRLZ; Coiled-coil domain-containing protein 88B; Brain leucine zipper domain-containing protein; Hook-related protein 3; HkRP3
Product Gene Name
CCDC88B sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for A6NC98
Specificity
CCDC88B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCDC88B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CCDC88B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CCDC88B sirna
siRNA to inhibit CCDC88B expression using RNA interference
Applications Tested/Suitable for CCDC88B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CCDC88B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_115627.6
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NCBI GenBank Nucleotide #
NM_032251.5
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UniProt Primary Accession #
A6NC98
[Other Products]
UniProt Secondary Accession #
Q05BL2; Q6RUV3; Q8N1Q6; Q8NF44; Q9H0H1; A5D8Y5; B5MDM2[Other Products]
UniProt Related Accession #
A6NC98[Other Products]
Molecular Weight
15,345 Da
NCBI Official Full Name
coiled-coil domain-containing protein 88B
NCBI Official Synonym Full Names
coiled-coil domain containing 88B
NCBI Official Symbol
CCDC88B [Similar Products]
NCBI Official Synonym Symbols
BRLZ; HKRP3; gipie; CCDC88
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NCBI Protein Information
coiled-coil domain-containing protein 88B
UniProt Protein Name
Coiled-coil domain-containing protein 88B
UniProt Synonym Protein Names
Brain leucine zipper domain-containing protein; Hook-related protein 3; HkRP3
Protein Family
Coiled-coil domain-containing protein
UniProt Gene Name
CCDC88B [Similar Products]
UniProt Synonym Gene Names
BRLZ; HkRP3 [Similar Products]
UniProt Entry Name
CC88B_HUMAN
NCBI Summary for CCDC88B
This gene encodes a member of the hook-related protein family. Members of this family are characterized by an N-terminal potential microtubule binding domain, a central coiled-coiled and a C-terminal Hook-related domain. The encoded protein may be involved in linking organelles to microtubules. [provided by RefSeq, Oct 2009]
UniProt Comments for CCDC88B
CCDC88B: a member of the hook-related protein family. Members of this family are characterized by an N-terminal potential microtubule binding domain, a central coiled-coiled and a C-terminal Hook-related domain. The encoded protein may be involved in linking organelles to microtubules. [provided by RefSeq, Oct 2009]
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 11q12.3
Cellular Component: membrane
Research Articles on CCDC88B
1. results demonstrate an important role for HkRP3 in regulating the clustering of lytic granules and MTOC repositioning during the development of NK cell-mediated killing.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
