ATPase, H+ transporting, lysosomal V0 subunit a4, ELISA Kit

Human V-type proton ATPase 116 kDa subunit a isoform 4 (ATP6V0A4) ELISA kit; tcag7.346; A4; ATP6N1B; ATP6N2; MGC130016; MGC130017; RDRTA2; RTA1C; RTADR; STV1; VPH1; VPP2; ATPase; H+ transporting; lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B; ATPase; H+; ATPase; H+ transporting; lysosomal V0 subunit a4

For Research Use Only. Not for use in diagnostic procedures.

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ATP6V0A4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9327390 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP6V0A4. The ELISA analytical biochemical technique of the MBS9327390 kit is based on ATP6V0A4 antibody-ATP6V0A4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP6V0A4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP6V0A4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ATP6V0A4 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

96,386 Da

ATPase, H+ transporting, lysosomal V0 subunit a4

V-type proton ATPase 116 kDa subunit a isoform 4; V-ATPase 116 kDa; vacuolar proton pump, subunit 2; vacuolar proton pump 116 kDa accessory subunit; vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform; H(+)-transporting two-sector ATPase, noncatalytic accessory protein 1B; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38kD)

V-type proton ATPase 116 kDa subunit a isoform 4

ATP6N1B; ATP6N2; V-ATPase 116 kDa isoform a4  [Similar Products]

VPP4_HUMAN

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]

ATP6V0A4: Part of the proton channel of the V-ATPase that is involved in normal vectorial acid transport into the urine by the kidney. Defects in ATP6V0A4 are the cause of distal renal tubular acidosis with preserved hearing (RTADR). RTADR is an autosomal recessive form of distal renal tubular acidosis (dRTA), a group of disorders characterized by functional failure of alpha- intercalated cells of the cortical collecting duct of the distal nephron, where vectorial proton transport is required for urinary acidification. Functional failure of alpha-intercalated cells results in metabolic acidosis accompanied by disturbances of potassium balance, urinary calcium solubility, bone physiology and growth. Belongs to the V-ATPase 116 kDa subunit family.

Protein type: Transporter, ion channel; Membrane protein, integral; Transporter; Energy Metabolism - oxidative phosphorylation; Membrane protein, multi-pass; Transporter, iron

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: phagocytic vesicle membrane; brush border membrane; apical part of cell; lysosomal membrane; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; vacuolar proton-transporting V-type ATPase complex; endosome

Molecular Function: protein binding; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding

Biological Process: vacuolar acidification; interaction with host; ATP synthesis coupled proton transport; ossification; proton transport; sensory perception of sound; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; regulation of pH; excretion; transmembrane transport

Disease: Renal Tubular Acidosis, Distal, Autosomal Recessive

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