Full Product Name
AK1 siRNA (Rat)
Product Synonym Names
Adenylate kinase isoenzyme 1; AK 1; ATP-AMP transphosphorylase 1; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; Myokinase
Product Gene Name
AK1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P39069
Specificity
AK1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AK1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AK1 sirna
siRNA to inhibit AK1 expression using RNA interference
Applications Tested/Suitable for AK1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AK1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_077325.2
[Other Products]
NCBI GenBank Nucleotide #
NM_024349.3
[Other Products]
UniProt Primary Accession #
P39069
[Other Products]
UniProt Secondary Accession #
Q5EBC5; Q9JJN3[Other Products]
UniProt Related Accession #
P39069[Other Products]
Molecular Weight
21,584 Da
NCBI Official Full Name
adenylate kinase isoenzyme 1
NCBI Official Synonym Full Names
adenylate kinase 1
NCBI Official Symbol
Ak1 [Similar Products]
NCBI Official Synonym Symbols
Ak 1
[Similar Products]
NCBI Protein Information
adenylate kinase isoenzyme 1
UniProt Protein Name
Adenylate kinase isoenzyme 1
UniProt Synonym Protein Names
ATP-AMP transphosphorylase 1
Protein Family
Adenylate kinase
UniProt Gene Name
Ak1 [Similar Products]
UniProt Synonym Gene Names
AK 1 [Similar Products]
UniProt Entry Name
KAD1_RAT
NCBI Summary for AK1
catalyzes the conversion of ATP and AMP to ADP in adenine nucleotide metabolism [RGD, Feb 2006]
UniProt Comments for AK1
AK1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency (HAAKD). Belongs to the adenylate kinase family.
Protein type: EC 2.7.4.3; Kinase, other; EC 2.7.4.6; Nucleotide Metabolism - purine
Cellular Component: sarcomere; neuron projection; perinuclear region of cytoplasm; cytoplasm; plasma membrane; outer dense fiber
Molecular Function: nucleoside diphosphate kinase activity; adenylate kinase activity; ATP binding; phosphotransferase activity, phosphate group as acceptor
Biological Process: response to drug; positive regulation of neuron maturation; muscle development; olfactory bulb development; ADP biosynthetic process; nucleoside diphosphate phosphorylation; response to testosterone stimulus; AMP metabolic process; response to estradiol stimulus; ATP metabolic process; neuron differentiation; nucleoside triphosphate biosynthetic process; cerebellum development; inosine biosynthetic process; response to activity; cell cycle arrest; adenine metabolic process; nucleotide phosphorylation
Research Articles on AK1
1. Data indicate that the beta-alanine administration was able to inhibit the enzyme pyruvate kinase, cytosolic creatine kinase, and adenylate kinase activities in cerebral cortex, and increase in cerebellum.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
