active Caspase-3, Polyclonal Antibody

Apopain precursor; Cysteine protease CPP32; ICE3; CASP-3; CPP-32; CPP32; Caspase-3; SCA-1; SREBP cleavage activity 1; Yama protein p17

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Ig

Rabbit
Source: Human

Affinity-chromatography

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Small volumes of anti-CASP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

WB: 1:500-1:2000
ICC: 1:50-1:100
IHC: 1:50-1:100

Western blot analysis of active Caspase-3 expression in Jurkat, C6 and Hela cell lysates using active Caspase-3 antibody at 1/1000 dilution.Predicted band size:17KDa.Observed band size:35, 32, 17KDa.

17kDa[Similar Products]

caspase 3

caspase-3

Caspase-3

CPP32; CASP-3; CPP-32; SCA-1  [Similar Products]

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Apoptosis; EC 3.4.22.56; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: cytosol; nucleoplasm; nucleus; plasma membrane

Molecular Function: aspartic-type endopeptidase activity; caspase activator activity; cysteine-type endopeptidase activity; peptidase activity; protein binding

Biological Process: apoptosis; caspase activation; caspase activation via cytochrome c; cell structure disassembly during apoptosis; DNA fragmentation during apoptosis; erythrocyte differentiation; keratinocyte differentiation; negative regulation of apoptosis; nerve growth factor receptor signaling pathway; neuron differentiation; platelet formation; proteolysis; regulation of macroautophagy

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