Bone Morphogenetic Protein 1, CUB2 Domain, Polyclonal Antibody

Anti -Bone Morphogenetic Protein 1, CUB2 Domain (BMP1, ProCollagen C-Endopeptidase, PCP, mTld)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 8; NC_000008.10 (22022653..22069839). Location: 8p21.3

Polyclonal

IgG

Rabbit

Reactive with Bone Morphogenic protein-1 (BMP-1, PCP, ProCollagen CPeptidase). Species Crossreactivity: Human

Affinity Purified
Peptide affinity purified.

Supplied as a liquid in PBS buffer, pH 7.4, with the addition of 0.01% sodium azide as preservative and 50% glycerol as cryoprotectant.

The undiluted antibody solution is stable for approximately 12 months at 40C. For longer storage, store at-200C. The solution is buffered from freezing at -20 degree C by 50% glycerol, but will freeze at-80 degree C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 40C with 0.05% Sodium Azide as preservative.

Small volumes of anti-BMP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

BMP-1 is an extracellular zinc endopeptidase of the Astacin family, which also includes Astacin, Meprin-A and B, Tolloid-like proteins and Choriolysin. The name BMP-1 reflects the involvement of PCP in the collagen deposition of growing bone. Procollagen C-peptidase cleaves the carboxy end from procollagen, to allow formation of mature, triplehelical collagen. The Aminoterminal end must also be cleaved by the Procollagen N-Proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP-1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP-1 have been reported, with varying truncation at the carboxyterminal end. The long form of BMP-1 is most similar to the Tolloid-like proteins, which have extra EGF-like and CUB domains. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

Antibodies; Abs to Collagen

Western Blot (WB)

Western blot: Starting dilutions 1:1000 for colorimetric substrates such as BCIP/NBT, 1:5000 for chemiluminescence substrates.

111,249 Da[Similar Products]

bone morphogenetic protein 1

bone morphogenetic protein 1; OTTHUMP00000123391; OTTHUMP00000224828; OTTHUMP00000224832; OTTHUMP00000224834; OTTHUMP00000227399; procollagen C-proteinase; mammalian tolloid protein; procollagen C-endopeptidase

Bone morphogenetic protein 1

PCOLC  [Similar Products]

BMP1_HUMAN

This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq]

BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.19; Cytokine; Protease

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: Golgi apparatus; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: peptidase activity; growth factor activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; cytokine activity; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; ossification; multicellular organismal development; lipoprotein metabolic process; proteolysis; cell differentiation; skeletal development; cartilage condensation

Disease: Osteogenesis Imperfecta, Type Xiii

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