DNA-Binding Protein SATB2 (SATB2), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of SATB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9367050 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA-Binding Protein SATB2 (SATB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SATB2. The ELISA analytical biochemical technique of the MBS9367050 kit is based on SATB2 antibody-SATB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SATB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SATB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

69,137 Da

SATB homeobox 2

DNA-binding protein SATB2

DNA-binding protein SATB2

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SATB2_HUMAN

This gene encodes a DNA binding protein that specifically binds nuclear matrix attachment regions. The encoded protein is involved in transcription regulation and chromatin remodeling. Defects in this gene are associated with isolated cleft palate and mental retardation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Feb 2010]

SATB2: Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper- layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11). Defects in SATB2 are a cause of cleft palate isolated (CPI). A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis. A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22). Belongs to the CUT homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cytoplasm; histone deacetylase complex; nuclear matrix; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; protein binding; sequence-specific DNA binding

Biological Process: cartilage development; chromatin remodeling; commitment of a neuronal cell to a specific type of neuron in the forebrain; embryonic pattern specification; embryonic skeletal morphogenesis; negative regulation of transcription from RNA polymerase II promoter; neuron migration; osteoblast development; palate development; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

Disease: Glass Syndrome

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