cAMP-dependent protein kinase catalytic subunit alpha, ELISA Kit

cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACA; PKACA; 2.7.11.11

For Research Use Only. Not for use in diagnostic procedures.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of PRKACA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2883609 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cAMP-dependent protein kinase catalytic subunit alpha (PRKACA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRKACA. The ELISA analytical biochemical technique of the MBS2883609 kit is based on PRKACA antibody-PRKACA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRKACA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRKACA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

39,822 Da

protein kinase cAMP-activated catalytic subunit alpha

cAMP-dependent protein kinase catalytic subunit alpha

cAMP-dependent protein kinase catalytic subunit alpha

PKACA; PKA C-alpha  [Similar Products]

KAPCA_HUMAN

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]

PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described.

Protein type: EC 2.7.11.11; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; PKA family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: AMP-activated protein kinase complex; cAMP-dependent protein kinase complex; centrosome; cytosol; membrane; mitochondrion; nucleoplasm; nucleus

Molecular Function: ATP binding; cAMP-dependent protein kinase activity; protein binding; protein kinase binding; protein serine/threonine/tyrosine kinase activity; ubiquitin protein ligase binding

Biological Process: activation of protein kinase A; blood coagulation; carbohydrate metabolic process; cytosolic calcium ion homeostasis; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; G2/M transition of mitotic cell cycle; gluconeogenesis; glucose metabolic process; innate immune response; mitotic cell cycle; nerve growth factor receptor signaling pathway; organelle organization and biogenesis; peptidyl-serine phosphorylation; phospholipase C activation; protein amino acid phosphorylation; regulation of heart rate; regulation of insulin secretion; regulation of osteoblast differentiation; regulation of protein binding; renal water homeostasis; signal transduction; sperm capacitation; stimulatory C-type lectin receptor signaling pathway; transmembrane transport; triacylglycerol catabolic process; water transport

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