Product Name
Adenosine Receptor A1 (ADORA1), Polyclonal Antibody
Full Product Name
Adenosine Receptor A1 Antibody: Dylight 633
Product Synonym Names
Rabbit Anti-Human Adenosine receptor A1 Polyclonal; ADORA1 Antibody; Adenosine receptor A1 Antibody; Adenosine A1 Receptor Antibody; A1AR Antibody; A1R Antibody; AA1R Antibody; ADORA 1 antibody; RDC 7 Antibody; RDC7 Antibody; Ri Antibody; Adenosine receptor (A1) Antibody
Product Gene Name
anti-ADORA1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P30542
Specificity
Detects ~37 kDa.
Purity/Purification
Peptide Affinity Purified
Form/Format
PBS, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide from the C-terminal of Human Adenosine receptor A1
Cellular Localization
Cell Membrane; Multi-Pass Membrane Protein
Certificate of Analysis
A 1:1000 dilution was sufficient for detection of Adenosine receptor A1 in 15 ug of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-ADORA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-ADORA1 antibody
Immunology; Innate Immunity; Macrophage; Cardiovascular System; Atherosclerosis; Vascular Inflammation; Inflammatory Mediators; Cardiovascular System; Heart; Cancer; Apoptosis
Applications Tested/Suitable for anti-ADORA1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-ADORA1 antibody
WB: 1:1000
ICC/IF: 1:100
IHC: 1:50
Immunocytochemistry (ICC) of anti-ADORA1 antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody. Tissue: Cerebellum. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~36.5 kDa.
NCBI/Uniprot data below describe general gene information for ADORA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000665.1
[Other Products]
NCBI GenBank Nucleotide #
NP_000665.1
[Other Products]
UniProt Primary Accession #
P30542
[Other Products]
UniProt Related Accession #
P30542[Other Products]
NCBI Official Full Name
adenosine receptor A1 isoform 1
NCBI Official Synonym Full Names
adenosine A1 receptor
NCBI Official Symbol
ADORA1 [Similar Products]
NCBI Official Synonym Symbols
RDC7
[Similar Products]
NCBI Protein Information
adenosine receptor A1
UniProt Protein Name
Adenosine receptor A1
Protein Family
Adenosine receptor
UniProt Gene Name
ADORA1 [Similar Products]
UniProt Entry Name
AA1R_HUMAN
NCBI Summary for ADORA1
The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ADORA1
ADORA1: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Apoptosis; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: asymmetric synapse; presynaptic membrane; postsynaptic membrane; cell soma; endoplasmic reticulum; basolateral plasma membrane; integral to plasma membrane; postsynaptic density; presynaptic active zone; plasma membrane; dendritic spine; terminal button; axolemma
Molecular Function: protein binding; G-protein-coupled receptor binding; protein heterodimerization activity; G-protein beta/gamma-subunit binding; purine nucleoside binding; adenosine receptor activity, G-protein coupled; phospholipase C activity; heterotrimeric G-protein binding
Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; relaxation of vascular smooth muscle; activation of MAPKK activity; negative regulation of glutamate secretion; negative regulation of hormone secretion; positive regulation of potassium ion transport; regulation of synaptic plasticity; positive regulation of peptide secretion; neurological control of breathing; thermoregulation; signal transduction; positive regulation of protein amino acid dephosphorylation; negative regulation of cell proliferation; regulation of glomerular filtration; cell-cell signaling; positive regulation of nucleoside transport; positive regulation of epidermal growth factor receptor activity; negative regulation of lipid catabolic process; negative regulation of vasodilation; negative regulation of blood pressure; detection of temperature stimulus involved in sensory perception of pain; protein targeting to membrane; inflammatory response; negative regulation of synaptic transmission, GABAergic; adenosine receptor signaling pathway; nervous system development; negative regulation of neurotrophin production; negative regulation of synaptic transmission, glutamatergic; negative regulation of acute inflammatory response; negative regulation of leukocyte migration; phagocytosis; response to hypoxia; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; cognition; positive regulation of blood pressure; lipid catabolic process; negative regulation of apoptosis
Research Articles on ADORA1
1. Abeta and tau may be considered as novel biomarkers of sleep disorder in AD pathology, and that they function by regulating the expression levels of orexin A and adenosine A1R.
Precautions
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