Three Prime Repair Exonuclease 1, Recombinant Protein

AGS1; ATRIP; DRN3; Aicardi-Goutieres Syndrome 1; 3'-5' exonuclease TREX1; DNase III

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 95%

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of TREX1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).

33,675 Da

Three-prime repair exonuclease 1

TREX1_MOUSE

TREX1: the major 3' DNA exonuclease in mammalian cells. Normally associates with the endoplasmic reticulum (ER). Translocates to the nucleus at S phase after DNA damage by gamma-irradiation or hydroxyurea. Trex1-deficient cells show defective G1/S transition, with single-stranded DNA molecules persisting after S phase and accumulating in the ER. Degrades ssDNA. Prevents chronic checkpoint activation and inappropriate immune activation. Mutations cause Aicardi-Goutieres syndrome, an autoimmune disorder. Trex1a(-/-) mice have autoinflammatory responses. The gene for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode TREX1 also encode ATRIP in another reading frame. Three alternatively spliced human isoforms have been reported.

Protein type: Deoxyribonuclease; DNA repair, damage; Cell cycle regulation; EC 3.1.11.2; DNA-binding

Cellular Component: cytoplasm; endoplasmic reticulum; membrane; nucleus

Molecular Function: 3'-5' exonuclease activity; 3'-5'-exodeoxyribonuclease activity; adenyl deoxyribonucleotide binding; double-stranded DNA binding; exodeoxyribonuclease III activity; exonuclease activity; hydrolase activity; metal ion binding; MutLalpha complex binding; MutSalpha complex binding; nuclease activity; nucleic acid binding; protein binding; protein homodimerization activity; single-stranded DNA binding

Biological Process: DNA metabolic process

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