SATB2, Polyclonal Antibody

SATB2; KIAA1034; DNA-binding protein SATB2; Special AT-rich sequence-binding protein 2; SATB homeobox 2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.

SATB2 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

SATB2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Small volumes of anti-SATB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SATB2 Antibody: Human special AT-rich sequence-binding protein-2 (SATB2) is a nuclear matrix/scaffold-associated region DNA-binding protein. Like its homolog SATB1, SATB2 selectively binds double-stranded, special AT-rich DNA sequences, but is expressed primarily in a subset of postmitotic, differentiating neurons in the neocortex. Mice deficient in SATB exhibit craniofacial abnormalities and defects in osteoblast differentiation and function. SATB2 also interacts with and enhances the activity of Runx2 and ATF4, two transcription factors that regulate osteoblast differentiation, indicating that SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Recent experiments have shown that SATB2 interacts with histone deacetylase 1 and metastasis-associated protein 2, two proteins that are involved in chromatin remodeling, suggesting that SATB2 may also be involved in mediating epigenetic influences during cortical development.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

SATB2 antibody can be used for detection of SATB2 by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western blot analysis of SATB2 in mouse brain tissue lysate with SATB2 antibody at (A) 2 and (B) 4 μg/mL.

Immunohistochemistry of SATB2 in human brain with SATB2 antibody at 5 μg/mL.

Immunofluorescence of SATB2 in Human Brain cells with SATB2 antibody at 20 μg/mL.

69,137 Da

SATB homeobox 2

DNA-binding protein SATB2; SATB family member 2; special AT-rich sequence-binding protein 2

DNA-binding protein SATB2

KIAA1034  [Similar Products]

SATB2_HUMAN

This gene encodes a DNA binding protein that specifically binds nuclear matrix attachment regions. The encoded protein is involved in transcription regulation and chromatin remodeling. Defects in this gene are associated with isolated cleft palate and mental retardation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Feb 2010]

SATB2: Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper- layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11). Defects in SATB2 are a cause of cleft palate isolated (CPI). A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis. A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22). Belongs to the CUT homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: nucleoplasm; transcription factor complex; nuclear matrix; histone deacetylase complex; cytoplasm

Molecular Function: protein binding; sequence-specific DNA binding; chromatin binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; commitment of a neuronal cell to a specific type of neuron in the forebrain; cartilage development; neuron migration; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; palate development; embryonic pattern specification; embryonic skeletal morphogenesis; osteoblast development

Disease: Glass Syndrome

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