Product Name
H3F3B, siRNA
Full Product Name
H3F3B siRNA (Rat)
Product Synonym Names
H3.3B; Histone H3.3
Product Gene Name
H3F3B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P84245
Specificity
H3F3B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat H3F3B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of H3F3B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
H3F3B sirna
siRNA to inhibit H3F3B expression using RNA interference
Applications Tested/Suitable for H3F3B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for H3F3B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_446437.1
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NCBI GenBank Nucleotide #
NM_053985.2
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UniProt Primary Accession #
P84245
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UniProt Secondary Accession #
P06351; P33155; Q5RJM5; Q9V3W4[Other Products]
UniProt Related Accession #
P84245[Other Products]
Molecular Weight
15,328 Da
NCBI Official Full Name
histone H3.3
NCBI Official Synonym Full Names
H3 histone, family 3B
NCBI Official Symbol
H3f3b [Similar Products]
NCBI Official Synonym Symbols
H3.3B
[Similar Products]
NCBI Protein Information
histone H3.3
UniProt Protein Name
Histone H3.3
UniProt Gene Name
H3f3b [Similar Products]
UniProt Synonym Gene Names
H3.3b [Similar Products]
UniProt Entry Name
H33_RAT
NCBI Summary for H3F3B
histone that may be involved in brain development [RGD, Feb 2006]
UniProt Comments for H3F3B
H3 iso3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
Protein type: DNA-binding
Cellular Component: nuclear chromosome; protein complex; nucleosome; nucleus
Molecular Function: nucleosomal DNA binding; DNA binding; protein heterodimerization activity
Biological Process: osteoblast differentiation; nucleosome assembly; muscle cell differentiation; DNA replication-independent nucleosome assembly; response to hormone stimulus; brain development; positive regulation of cell growth; nuclear organization and biogenesis
Research Articles on H3F3B
1. Data indicate that histone demethylases KDM4A/C regulate H3K9 and H3K36 methylation on brain-derived neurotrophic factor (BDNF) versus astroglial glial fibrillary acidic protein (GFAP) genes in neural stem cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
