Product Name
KCNQ1, siRNA
Full Product Name
KCNQ1 siRNA (Mouse)
Product Synonym Names
KCNA9; KVLQT1; Potassium voltage-gated channel subfamily KQT member 1; IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1; KQT-like 1; Voltage-gated potassium channel subunit Kv7.1
Product Gene Name
KCNQ1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P97414
Specificity
KCNQ1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KCNQ1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KCNQ1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KCNQ1 sirna
siRNA to inhibit KCNQ1 expression using RNA interference
Applications Tested/Suitable for KCNQ1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KCNQ1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032460.2
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NCBI GenBank Nucleotide #
NM_008434.2
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UniProt Primary Accession #
P97414
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UniProt Secondary Accession #
O88702; Q3U4H1; Q7TNZ1; Q7TPL7; Q80VR7[Other Products]
UniProt Related Accession #
P97414[Other Products]
Molecular Weight
62,640 Da
NCBI Official Full Name
potassium voltage-gated channel subfamily KQT member 1
NCBI Official Synonym Full Names
potassium voltage-gated channel, subfamily Q, member 1
NCBI Official Symbol
Kcnq1 [Similar Products]
NCBI Official Synonym Symbols
Kcna9; KVLQT1; AW559127
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NCBI Protein Information
potassium voltage-gated channel subfamily KQT member 1
UniProt Protein Name
Potassium voltage-gated channel subfamily KQT member 1
UniProt Synonym Protein Names
IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1; KQT-like 1; Voltage-gated potassium channel subunit Kv7.1
Protein Family
KCNQ1 downstream neighbor protein
UniProt Gene Name
Kcnq1 [Similar Products]
UniProt Synonym Gene Names
Kcna9; Kvlqt1 [Similar Products]
UniProt Entry Name
KCNQ1_MOUSE
UniProt Comments for KCNQ1
Kv7.1: a voltage-gated potassium channel protein required for the repolarization phase of the cardiac action potential. Associates with KCNE1 (MinK) to form the I(Ks) cardiac potassium current. Elicits a rapidly activating, potassium-selective outward current. May associate also with KCNE3 (MiRP2) to form the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions, which is reduced in cystic fibrosis and pathologically stimulated in cholera and other forms of secretory diarrhea. Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries. Three alternatively spliced isoforms have been described.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, potassium
Cellular Component: voltage-gated potassium channel complex; zymogen granule membrane; cytoplasmic vesicle membrane; membrane; lysosome; integral to plasma membrane; basolateral plasma membrane; early endosome; late endosome; integral to membrane; plasma membrane; cytoplasmic vesicle; sarcolemma
Molecular Function: calmodulin binding; voltage-gated potassium channel activity; protein homodimerization activity; potassium channel activity; outward rectifier potassium channel activity; delayed rectifier potassium channel activity; ion channel activity; voltage-gated ion channel activity; protein phosphatase 1 binding
Biological Process: regulation of membrane potential; genetic imprinting; transport; ion transport; positive regulation of heart rate; gene silencing; transmembrane transport; potassium ion transport; negative regulation of insulin secretion; cardiac muscle contraction
Research Articles on KCNQ1
1. Characterization of the imprinted Kcnq1 domain which contains a differentially methylated region in intron 11 of Kcnq1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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