Full Product Name
CAV2 siRNA (Rat)
Product Synonym Names
Caveolin-2
Product Gene Name
CAV2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q2IBC5
Specificity
CAV2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CAV2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CAV2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CAV2 sirna
siRNA to inhibit CAV2 expression using RNA interference
Applications Tested/Suitable for CAV2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CAV2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_571989.2
[Other Products]
NCBI GenBank Nucleotide #
NM_131914.2
[Other Products]
UniProt Primary Accession #
Q2IBC5
[Other Products]
UniProt Secondary Accession #
Q6P6R8; Q8VIK7[Other Products]
UniProt Related Accession #
Q2IBC5[Other Products]
Molecular Weight
18,266 Da
NCBI Official Full Name
caveolin-2
NCBI Official Synonym Full Names
caveolin 2
NCBI Official Symbol
Cav2 [Similar Products]
NCBI Protein Information
caveolin-2
UniProt Protein Name
Caveolin-2
UniProt Gene Name
Cav2 [Similar Products]
UniProt Entry Name
CAV2_RAT
NCBI Summary for CAV2
a component of caveolae; involved in cholesterol transport, signal transduction, and transcytosis, may also function as a SNARE accessory protein [RGD, Feb 2006]
UniProt Comments for CAV2
Caveolin-2: a scaffolding protein. A component of caveolae, small invaginations of the plasma membrane found in most cell types. Involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Caveolin-2 may function as an accessory protein in conjunction with caveolin-1. Two splice-variant isoforms have been observed.
Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold
Cellular Component: Golgi apparatus; protein complex; focal adhesion; cell surface; integral to plasma membrane; endoplasmic reticulum; nuclear inner membrane; lipid particle; nuclear envelope; caveola; cytosol; acrosomal membrane; lipid raft; Golgi membrane; transport vesicle; extrinsic to internal side of plasma membrane; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; intracellular; cytoplasmic vesicle
Molecular Function: protein binding, bridging; protein binding; SNARE binding; protein homodimerization activity; syntaxin binding; protein heterodimerization activity; phosphoprotein binding; mitogen-activated protein kinase kinase kinase binding; protein complex scaffold; D1 dopamine receptor binding; protein kinase binding
Biological Process: endoplasmic reticulum organization and biogenesis; positive regulation of dopamine receptor signaling pathway; mitochondrion organization and biogenesis; vesicle organization and biogenesis; skeletal muscle fiber development; protein oligomerization; vesicle fusion; negative regulation of cell proliferation; synaptic transmission; positive regulation of peptidyl-tyrosine phosphorylation; vesicle docking; positive regulation of MAPKKK cascade; negative regulation of endothelial cell proliferation; insulin receptor signaling pathway; regulation of mitosis; positive regulation of endothelial cell proliferation; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of GTPase activity
Research Articles on CAV2
1. show that Cav-2 is an epigenetic regulator of histone H3 modifications, and provide novel mechanisms of insulin-response epigenetic activation at the nuclear periphery
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
