Actin-related protein 2/3 complex subunit 2, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Caprylic Acid Ammonium Sulfate Precipitation

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-ARPC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ARPC2 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of ARPC2, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

ELISA (EIA)

Western blot Actin-related protein 2/3 complex subunit 2 Antibody at 2 /ml SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 33kDaObserved band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.

34,333 Da[Similar Products]

actin related protein 2/3 complex subunit 2

actin-related protein 2/3 complex subunit 2

Actin-related protein 2/3 complex subunit 2

ARC34; p34-ARC  [Similar Products]

ARPC2_HUMAN

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

ARPC2: Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. Belongs to the ARPC2 family.

Protein type: Actin-binding; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: actin cytoskeleton; Arp2/3 protein complex; cytoplasm; cytosol; endosome; focal adhesion; Golgi apparatus; leading edge; neuron projection; synapse

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton

Biological Process: axon guidance; cell motility; ephrin receptor signaling pathway; innate immune response; small GTPase mediated signal transduction

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