Nephrin, ELISA Kit

Nephrin; Renal glomerulus-specific cell adhesion receptor; Nphs1; Nphn

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C

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Small volumes of Nphs1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9426300 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nephrin (Nphs1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Nphs1. The ELISA analytical biochemical technique of the MBS9426300 kit is based on Nphs1 antibody-Nphs1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Nphs1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Nphs1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

126,646 Da

NPHS1 nephrin

nephrin

Nephrin

Nphn  [Similar Products]

renal protein localized specifically to glomerular capillary walls; may contribute to the development of proteinuria in renal disease [RGD, Feb 2006]

NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cell projection; lipid raft; membrane; plasma membrane; protein complex

Molecular Function: alpha-actinin binding; myosin binding; protein binding; protein domain specific binding; spectrin binding

Biological Process: cell adhesion; glomerular basement membrane development; glomerular filtration; JNK cascade; MAPKKK cascade; myoblast fusion; positive regulation of actin filament polymerization; skeletal muscle development

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