Claudin 16, Polyclonal Antibody

Anti -Claudin 16 (Paracellin 1, CLDN16)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 3; NC_000003.11 (190105661..190129932). Location: 3q28

Polyclonal

IgG

Rabbit

Recognizes human Paracellin-1. Species Sequence Homology: bovine (70%)

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-CLDN16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Magnesium as a cofactor is required in many cellular activities. Mg+2 reabsorption in the kidney is mediated primarily be a poorly understood paracellular pathway (passage of solutes between the cells) in the thick ascending limb of Henle (TAL). Tight junctions constitute the barrier to paracellular conductance. Familial hypomagnesaemia with hypercalciuria and nephrocalcinosis (FHHNC, MIM 248250) is a complex renal tubular disorder characterized by hypomagnesaemia, hypercalciuria, advanced nephrocalcinL6060606osis, hyposthenuria and progressive renal failure. The mode of inheritance is autosomal recessive. A primary defect in the reabsorption of magnesium in the TAL has been proposed to be essential in FHHNC pathophysiology. Recently, mutations in the gene paracellin-1 (PCLN-1/Claudin-16) have been identified as the underlying genetic defect in FHHNC. Null mutation of PCLN-1/Claudin-16 has been shown to produce chronic interstitial nephritis in cattle. PCLN-1 gene codes for a protein of 305 aa (chromosome 3q27), with four TM domains and intracellular NH2- and COOH-termini. PCLN-1 belongs to the Claudin family of proteins. It is 10-18% related with claudins. PCLN-1 is only expressed in tight junction of TAL implicated in Mg+2-reabsorption.

Antibodies; Abs to Ion Channel

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000-:1:3000 using ECL technique.
ELISA: 0.5-1ug/ml for affinity pure.

33,836 Da[Similar Products]

claudin 16

claudin-16; PCLN-1; paracellin-1; OTTHUMP00000209499; hypomagnesemia 3, with hypercalciuria and nephrocalcinosis

Claudin-16

PCLN1  [Similar Products]

CLD16_HUMAN

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28. [provided by RefSeq]

Claudin-16: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Defects in CLDN16 are the cause of hypomagnesemia type 3 (HOMG3); also known as familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC). HOMG3 is a progressive renal disease characterized by primary renal magnesium wasting with hypomagnesemia, hypercalciuria and nephrocalcinosis. Recurrent urinary tract infections and kidney stones are often observed. In spite of hypercalciuria, patients do not show hypocalcemia. Belongs to the claudin family.

Protein type: Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: tight junction; integral to membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; magnesium ion transmembrane transporter activity; structural molecule activity

Biological Process: cellular metal ion homeostasis; excretion; magnesium ion transport; calcium-independent cell-cell adhesion

Disease: Hypomagnesemia 3, Renal

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