Product Name
ASCC1, siRNA
Full Product Name
ASCC1 siRNA (Human)
Product Synonym Names
Activating signal cointegrator 1 complex subunit 1; ASC-1 complex subunit p50; Trip4 complex subunit p50
Product Gene Name
ASCC1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8N9N2
Specificity
ASCC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ASCC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ASCC1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ASCC1 sirna
siRNA to inhibit ASCC1 expression using RNA interference
Applications Tested/Suitable for ASCC1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ASCC1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001185727.1
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NCBI GenBank Nucleotide #
NM_001198798.2
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UniProt Primary Accession #
Q8N9N2
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UniProt Secondary Accession #
Q5SW06; Q5SW07; Q96EI8; Q9Y307[Other Products]
UniProt Related Accession #
Q8N9N2[Other Products]
Molecular Weight
41,228 Da
NCBI Official Full Name
activating signal cointegrator 1 complex subunit 1 isoform b
NCBI Official Synonym Full Names
activating signal cointegrator 1 complex subunit 1
NCBI Official Symbol
ASCC1 [Similar Products]
NCBI Official Synonym Symbols
p50; CGI-18; ASC1p50
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NCBI Protein Information
activating signal cointegrator 1 complex subunit 1
UniProt Protein Name
Activating signal cointegrator 1 complex subunit 1
UniProt Synonym Protein Names
ASC-1 complex subunit p50; Trip4 complex subunit p50
Protein Family
Activating signal cointegrator 1 complex
UniProt Gene Name
ASCC1 [Similar Products]
UniProt Entry Name
ASCC1_HUMAN
NCBI Summary for ASCC1
This gene encodes a subunit of the activating signal cointegrator 1 (ASC-1) complex. The ASC-1 complex is a transcriptional coactivator that plays an important role in gene transactivation by multiple transcription factors including activating protein 1 (AP-1), nuclear factor kappa-B (NF-kB) and serum response factor (SRF). The encoded protein contains an N-terminal KH-type RNA-binding motif which is required for AP-1 transactivation by the ASC-1 complex. Mutations in this gene are associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
UniProt Comments for ASCC1
ASCC1: Enhances NF-kappa-B, SRF and AP1 transactivation. In cells responding to gastrin-activated paracrine signals, it is involved in the induction of SERPINB2 expression by gastrin. Defects in ASCC1 may be a cause of Barrett esophagus (BE). A condition characterized by a metaplastic change in which normal esophageal squamous epithelium is replaced by a columnar and intestinal-type epithelium. Patients with Barrett esophagus have an increased risk of esophageal adenocarcinoma. The main cause of Barrett esophagus is gastroesophageal reflux. The retrograde movement of acid and bile salts from the stomach into the esophagus causes prolonged injury to the esophageal epithelium and induces chronic esophagitis, which in turn is believed to trigger the pathologic changes. Genetic variants in ASCC1 have been found in individuals with Barrett esophagus and are thought to contribute to disease susceptibility. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10pter-q25.3
Cellular Component: transcription factor complex; cytoplasm
Molecular Function: RNA binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Disease: Barrett Esophagus
Research Articles on ASCC1
1. Three major genes, MSR1, ASCC1, and CTHRC1 were associated with Barrett esophagus/esophageal adenocarcinoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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