Bullous Pemhpigoid, ELISA Kit

Rat Bullous Pemhpigoid ELISA Kit; Bullous Pemhpigoid; Bullous Pemhpigoid (Rat)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 6; NC_000006.11 (56322785..56507694, complement). Location: 6p12.1

Store all reagents at 2-8 degree C

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of BP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS729622 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bullous Pemhpigoid (BP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BP. The ELISA analytical biochemical technique of the MBS729622 kit is based on BP antibody-BP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Rat ELISA Kit

860,662 Da

dystonin

bullous pemphigoid antigen 1; trabeculin-beta; dystonia musculorum protein; hemidesmosomal plaque protein; bullous pemphigoid antigen 1, 230/240kDa

Dystonin

BP230; BP240; BPAG1; DMH; DT; KIAA0728; BPA  [Similar Products]

DYST_HUMAN

This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of some variants has not been defined. It has been reported that some isoforms are expressed in neural and muscle tissue, anchoring neural intermediate filaments to the actin cytoskeleton, and some isoforms are expressed in epithelial tissue, anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression, mice defective for this gene show skin blistering and neurodegeneration. [provided by RefSeq, Mar 2010]

BPAG1 iso3: a protein of the plakin family. Plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. Ten alternatively spliced human isoforms have been reported. BPAG1 has neuronal and muscle isoforms that consist of actin-binding and microtubule-binding domains at either end separated by a plakin domain and many spectrin repeats. Bpag1 isoforms with different N-termini may have differing roles. The plakin domain contains an apparent nuclear localization signal.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Microtubule-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: endoplasmic reticulum membrane; intermediate filament cytoskeleton; focal adhesion; leading edge; cytoplasmic membrane-bound vesicle; integral to membrane; intermediate filament; nuclear envelope; cell cortex; cytosol; Z disc; actin cytoskeleton; microtubule cytoskeleton; hemidesmosome; axon; cytoplasm; basal plasma membrane; basement membrane; nucleus

Molecular Function: protein C-terminus binding; integrin binding; microtubule plus-end binding; protein binding; protein homodimerization activity; calcium ion binding; actin binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; hemidesmosome assembly; response to wounding; cell motility involved in cell locomotion; intermediate filament cytoskeleton organization and biogenesis; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; maintenance of cell polarity; cell cycle arrest; cell adhesion

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