Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7), ELISA Kit

CDw328; CD328; p75; AIRM1; D-Siglec; QA79; SIGLEC-7; P75/AIRM1; Adhesion inhibitory receptor molecule 1; QA79 membrane protein

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7).
No significant cross-reactivity or interference between Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of SIGLEC7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2703697 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SIGLEC7. The ELISA analytical biochemical technique of the MBS2703697 kit is based on SIGLEC7 antibody-SIGLEC7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SIGLEC7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SIGLEC7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

16,740 Da

sialic acid binding Ig like lectin 7

sialic acid-binding Ig-like lectin 7

Sialic acid-binding Ig-like lectin 7

AIRM1; Siglec-7; AIRM-1  [Similar Products]

SIGLEC7: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: integral component of plasma membrane; plasma membrane

Molecular Function: carbohydrate binding; receptor activity

Biological Process: cell adhesion; regulation of immune response

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