Full Product Name
anti-human CD23-R-PE
Product Synonym Names
Monoclonal anti-human CD23/R-PE
Product Gene Name
anti-CD23 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q63097
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 0.2 ml (lot specific)
Performance
Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated 5 minutes with 20 ul of 250ug/ml human IgG (to block non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD23/R-PE at a 1:50 dilution (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 13.6% sub-population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to a Mouse IgG1/PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD23 antibody.
Production
Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5 mg/ml with an A565/A280 of 3.12.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human peripheral myeloma cells
Preparation and Storage
Store at 2 - 5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Other Notes
Small volumes of anti-CD23 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD23 antibody
Information: Human CD23 is a low affinity receptor for IgE (Fc epsilon RII) found primarily on mature B cells and also on eosinophils, monocytes, dendritic cells, platelets, and a subset of thymic epithelial cells. CD23 interacts with CD21. Antibody BU38 recognizes the CD23 molecule of 45 kd.
Applications Tested/Suitable for anti-CD23 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), FPS, Agonist
Application Notes for anti-CD23 antibody
1:50 (or use 1.6 ul of concentrated stock per 5 x 105-cell test)
Testing Data of anti-CD23 antibody
NCBI/Uniprot data below describe general gene information for CD23. It may not necessarily be applicable to this product.
NCBI Accession #
CAA51981.1
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UniProt Primary Accession #
Q63097
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Molecular Weight
35,126 Da
NCBI Official Full Name
CD23
NCBI Official Synonym Full Names
Fc fragment of IgE, low affinity II, receptor for (CD23)
NCBI Official Symbol
Fcer2 [Similar Products]
NCBI Official Synonym Symbols
CD23; Fcer2a
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NCBI Protein Information
low affinity immunoglobulin epsilon Fc receptor; Fcalpha RII; Fcalpha muR; Fc receptor alpha multiple ligand receptor; Fc receptor, IgE, low affinity II, alpha polypeptide
UniProt Protein Name
CD23 protein
UniProt Synonym Protein Names
CD23 protein
UniProt Gene Name
Fcer2 [Similar Products]
UniProt Entry Name
Q63097_RAT
NCBI Summary for CD23
cell surface receptor; binds innumoglobulin (Ig)E and IgA antibodies [RGD, Feb 2006]
Product References and Citations for anti-CD23 antibody
Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 67-81. D. L. Hardie,
et al, (1993) Eur J Immunol 23: 997-1004. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 530-538. J.Y. Bonnefoy, et al, (1995) Curr Opin Immunol 7: 355-359.
Research Articles on CD23
1. Immunohistochemical analyses suggested that the Fcalpha/muR isoform was expressed in the tubular epithelial cells, but not in the glomeruli.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
