proteinase 3, ELISA Kit

Mouse Myeloblastin (PRTN3) ELISA kit; ACPA; AGP7; C-ANCA; CANCA; MBN; MBT; NP4; P29; PR-3; PR3; Wegener granulomatosis autoantigen; azurophil granule protein 7; leukocyte proteinase 3; myeloblastin; serine proteinase; neutrophil; proteinase 3

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse PRTN3. No significant cross-reactivity or interference between mouse PRTN3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PRTN3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS905144 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the proteinase 3 (PRTN3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRTN3. The ELISA analytical biochemical technique of the MBS905144 kit is based on PRTN3 antibody-PRTN3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRTN3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRTN3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRTN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRTN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRTN3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRTN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

27,626 Da

proteinase 3

myeloblastin; PR-3

Myeloblastin

PR-3  [Similar Products]

PRTN3_MOUSE

PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Protease; Cell cycle regulation; EC 3.4.21.76

Cellular Component: extracellular matrix; extracellular space; plasma membrane; cytosol

Molecular Function: peptidase activity; enzyme binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: negative regulation of phagocytosis; collagen catabolic process; proteolysis

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