Product Name
Ketohexokinase (KHK), Antibody
Full Product Name
Goat anti-Ketohexokinase Antibody
Product Synonym Names
KHK; ketohexokinase (fructokinase); hepatic fructokinase; ketohexokinase
Product Gene Name
anti-KHK antibody
[Similar Products]
Antibody/Peptide Pairs
KHK peptide (MBS428838) is used for blocking the activity of Ketohexokinase antibody (MBS423800)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P50053
Species Reactivity
Human, Mouse, Rat, Pig
Expected from sequence similarity: Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen Sequence
Peptide with sequence RCLSQRWQRGGNASN, from the internal region of the protein sequence according to NP_000212.1; NP_006479.1.
Peptide Sequence + C
RCLSQRWQRGGNASN
Note
This antibody is expected to recognize both reported isoforms (NP_000212.1; NP_006479.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-KHK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-KHK antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-KHK antibody
Pep-ELISA: antibody detection limit dilution 1:128000.
WB: Approx 30kDa band observed in Human, Mouse, Rat and Pig Liver lysates. Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry (IHC) of anti-KHK antibody
(0.03ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Liver lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for KHK. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000212.1
[Other Products]
NCBI Related Accession #
Manufactured in an ISO 9001:2008 Certified Laboratory.NP_006479.1; NP_114061.1; XP_003125348.1; NP_032465.2[Other Products]
NCBI GenBank Nucleotide #
NM_000221.2
[Other Products]
UniProt Primary Accession #
P50053
[Other Products]
UniProt Secondary Accession #
Q6IBK2; Q99532; Q9BRJ3; Q9UMN1[Other Products]
UniProt Related Accession #
P50053[Other Products]
NCBI Official Full Name
ketohexokinase isoform a
NCBI Official Synonym Full Names
ketohexokinase
NCBI Official Symbol
KHK [Similar Products]
NCBI Protein Information
ketohexokinase
UniProt Protein Name
Ketohexokinase
UniProt Synonym Protein Names
Hepatic fructokinase
Protein Family
Ketohexokinase
UniProt Gene Name
KHK [Similar Products]
NCBI Summary for KHK
This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for KHK
Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
Research Articles on KHK
1. Data indicate metabolic enzymes NAD kinase and ketohexokinase as candidate metabolic gene targets, and the chromatin remodeling protein INO80C as a tumor suppressor in KRAS(MUT) colorectal tumor xenograft.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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