Full Product Name
Anti-MiTF Rabbit pAb
Product Synonym Names
Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32; MITF; BHLHE32
Product Gene Name
anti-MiTF antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O75030
Species Reactivity
Human, Mouse, Rat
Specificity
MiTF Antibody detects endogenous levels of total MiTF
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
A synthesized peptide derived from human MiTF
Subcellular Location
Nucleus
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Other Notes
Small volumes of anti-MiTF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MiTF antibody
Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that is most widely known for its roles in melanocyte, ophthalmic, and osteoclast development. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Applications Tested/Suitable for anti-MiTF antibody
Western Blot (WB)
Application Notes for anti-MiTF antibody
WB: 1:500-1:2000
Western Blot (WB) of anti-MiTF antibody
Western blot analysis of MiTF expression in A375 cell lysate.
NCBI/Uniprot data below describe general gene information for MiTF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000239.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000248.3
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UniProt Primary Accession #
O75030
[Other Products]
UniProt Secondary Accession #
Q14841; Q9P2V0; Q9P2V1; Q9P2V2; Q9P2Y8; B4DJL2; D3K197; E9PFN0[Other Products]
UniProt Related Accession #
O75030[Other Products]
NCBI Official Full Name
microphthalmia-associated transcription factor isoform 4
NCBI Official Synonym Full Names
melanogenesis associated transcription factor
NCBI Official Symbol
MITF [Similar Products]
NCBI Official Synonym Symbols
MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
[Similar Products]
NCBI Protein Information
microphthalmia-associated transcription factor
UniProt Protein Name
Microphthalmia-associated transcription factor
UniProt Synonym Protein Names
Class E basic helix-loop-helix protein 32; bHLHe32
Protein Family
Microphthalmia-associated transcription factor
UniProt Gene Name
MITF [Similar Products]
UniProt Synonym Gene Names
BHLHE32; bHLHe32 [Similar Products]
NCBI Summary for MiTF
The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]
UniProt Comments for MiTF
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Research Articles on MiTF
1. demonstrate that the FANC pathway acts downstream MiTF and establish the existence of an epistatic relationship between MiTF and the FANC pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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