Full Product Name
Anti-CD23 Picoband antibody
Product Synonym Names
Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CD23; Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; FCER2; CD23A; CLEC4J; FCE2; IGEBF
Product Gene Name
anti-CD23 antibody
[Similar Products]
Product Synonym Gene Name
FCER2[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P06734
Species Reactivity
Human, Rat
No cross reactivity with other proteins.
Immunogen
E Coli-derived human CD23 recombinant protein (Position: D48-R284).
Subcellular Localization
Cell membrane; Also exists as a soluble excreted form, sCD23.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-CD23 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD23 antibody
Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
Applications Tested/Suitable for anti-CD23 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-CD23 antibody
WB: 0.1-0.5mug/ml
Direct ELISA: 0.1-0.5mug/ml
Western Blot (WB) of anti-CD23 antibody
Figure 1. Western blot analysis of CD23 using anti-CD23 antibody (MBS1750866).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD23 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD23 at approximately 36KD. The expected band size for CD23 is at 36KD.
NCBI/Uniprot data below describe general gene information for CD23. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001193948.2
[Other Products]
NCBI GenBank Nucleotide #
NP_001193948.2
[Other Products]
UniProt Primary Accession #
P06734
[Other Products]
UniProt Related Accession #
P06734[Other Products]
Molecular Weight
36,469 Da
NCBI Official Full Name
low affinity immunoglobulin epsilon Fc receptor isoform b
NCBI Official Synonym Full Names
Fc fragment of IgE receptor II
NCBI Official Symbol
FCER2 [Similar Products]
NCBI Official Synonym Symbols
CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
[Similar Products]
NCBI Protein Information
low affinity immunoglobulin epsilon Fc receptor
UniProt Protein Name
Low affinity immunoglobulin epsilon Fc receptor
UniProt Synonym Protein Names
BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CD_antigen: CD23
UniProt Gene Name
FCER2 [Similar Products]
UniProt Synonym Gene Names
CD23A; CLEC4J; FCE2; IGEBF [Similar Products]
NCBI Summary for CD23
The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
UniProt Comments for CD23
Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
Research Articles on CD23
1. Report a variant of t(14;18) negative nodal diffuse follicular lymphoma with CD23 expression, 1p36/TNFRSF14 abnormalities, and STAT6 mutations.
Precautions
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Disclaimer
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