Caveolin 3 (CAV-3), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store at 2 to 8 degree C for 6 months.

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Small volumes of CAV-3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1600527 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Caveolin 3 (CAV-3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CAV-3. The ELISA analytical biochemical technique of the MBS1600527 kit is based on CAV-3 antibody-CAV-3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CAV-3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CAV-3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Caveolin 3 (also known as CAV-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CAV-3 antibody. CAV-3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CAV-3 Antibody is added and binds to CAV-3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CAV-3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CAV-3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

17,259 Da

caveolin 3

caveolin-3

Caveolin-3

This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq, Jul 2008]

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (). Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae (PubMed:19262564).

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