Full Product Name
Dyskerin Antibody
Product Synonym Names
CBF5 homolog; DKC1; EC 5.4.99.-; Nucleolar protein NAP57
Product Gene Name
anti-DKC1 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O60832
Species Reactivity
Human, Mouse, Rat
Specificity
Dyskerin antibody detects endogenous levels of total Dyskerin protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized peptide derived from Internal of human Dyskerin.
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-DKC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-DKC1 antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-DKC1 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-DKC1 antibody
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:10000
Testing Data of anti-DKC1 antibody
Western Blot (WB) of anti-DKC1 antibody
Peptide-+ Western blot analysis of extracts from HeLa cells, using Dyskerin antibody. Immunofluorescence analysis of HeLa cells, using Dyskerin antibody.
NCBI/Uniprot data below describe general gene information for DKC1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001135935.1
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NCBI GenBank Nucleotide #
NM_001142463.2
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UniProt Primary Accession #
O60832
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UniProt Secondary Accession #
O43845; Q96G67; Q9Y505; F5BSB3[Other Products]
UniProt Related Accession #
O60832[Other Products]
Molecular Weight
47,603 Da[Similar Products]
NCBI Official Full Name
H/ACA ribonucleoprotein complex subunit 4 isoform 2
NCBI Official Synonym Full Names
dyskerin pseudouridine synthase 1
NCBI Official Symbol
DKC1 [Similar Products]
NCBI Official Synonym Symbols
DKC; CBF5; DKCX; NAP57; NOLA4; XAP101
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NCBI Protein Information
H/ACA ribonucleoprotein complex subunit 4
UniProt Protein Name
H/ACA ribonucleoprotein complex subunit 4
UniProt Synonym Protein Names
CBF5 homolog; Dyskerin; Nopp140-associated protein of 57 kDa; Nucleolar protein NAP57; Nucleolar protein family A member 4; snoRNP protein DKC1
UniProt Gene Name
DKC1 [Similar Products]
UniProt Synonym Gene Names
NOLA4 [Similar Products]
NCBI Summary for DKC1
This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Comments for DKC1
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Product References and Citations for anti-DKC1 antibody
Heiss N.S., Nat. Genet. 19:32-38(1998). Knight S.W., Am. J. Hum. Genet. 65:50-58(1999). Hassock S., Genomics 55:21-27(1999).
Research Articles on DKC1
1. dyskerin suppression caused p53-dependent G1 cell-cycle arrest in p53 wild-type cells, and a p53-independent pathway impaired proliferation in cells with p53 dysfunction.
Precautions
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