Product Name
AHSG / Fetuin, Polyclonal Antibody
Popular Item
Full Product Name
Goat Polyclonal to Human AHSG / Fetuin
Product Synonym Names
Anti-AHSG / Fetuin Antibody IHC-plus; A2HS; Alpha-2-Z-globulin; Fetuin; Fetuin-A; AHS; Alpha-2-HS-glycoprotein; Ba-alpha-2-glycoprotein; FETUA; HSGA; Human AHSG; Fetuin
Product Gene Name
anti-AHSG antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P02765
Specificity
Recombinant human fetuin (a2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.
Purity/Purification
Purified IgG
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
Concentration
1 mg/ml (lot specific)
Host Note
AHSG / Fetuin A antibody was produced in Goat
Immunogen Species
AHSG / Fetuin A antibody was raised against Human
Antigen Type
Recombinant protein
Immunogen
AHSG / Fetuin A antibody was raised against recombinant human AHSG / Fetuin A
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:
Tissue Preparation:
Formalin fixation and embedding in paraffin wax.
Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.
Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT
Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT
Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT
Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT
Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Preparation and Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Other Notes
Small volumes of anti-AHSG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AHSG antibody
Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue.
Applications Tested/Suitable for anti-AHSG antibody
Immunohistochemistry - Paraffin (IHC-P), Western Blot (WB), Immmunoprecipitation (IP), ELISA (EIA)
Optimal dilution to be determined by the researcher.
Application Notes for anti-AHSG antibody
IHC-Paraffin: 2.5 ug/ml
WB: 1:10000-1:20000
Summary: Immunohistochemistry: MBS240601 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS240601 was determined to be 2.5 ug/ml.
NCBI/Uniprot data below describe general gene information for AHSG. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001613.2
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NCBI GenBank Nucleotide #
NM_001622.2
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UniProt Primary Accession #
P02765
[Other Products]
UniProt Secondary Accession #
O14961; O14962; Q9P152; A8K9N6; B2R7G1[Other Products]
UniProt Related Accession #
P02765; AAB29984[Other Products]
NCBI Official Full Name
alpha-2-HS-glycoprotein preproprotein
NCBI Official Synonym Full Names
alpha-2-HS-glycoprotein
NCBI Official Symbol
AHSG [Similar Products]
NCBI Official Synonym Symbols
AHS; A2HS; HSGA; FETUA
[Similar Products]
NCBI Protein Information
alpha-2-HS-glycoprotein; fetuin-A; alpha-2-Z-globulin; ba-alpha-2-glycoprotein
UniProt Protein Name
Alpha-2-HS-glycoprotein
UniProt Synonym Protein Names
Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; Fetuin-ACleaved into the following 2 chains:Alpha-2-HS-glycoprotein chain A; Alpha-2-HS-glycoprotein chain B
Protein Family
Alpha-2-HS-glycoprotein
UniProt Gene Name
AHSG [Similar Products]
UniProt Synonym Gene Names
FETUA [Similar Products]
UniProt Entry Name
FETUA_HUMAN
NCBI Summary for AHSG
Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008]
UniProt Comments for AHSG
FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3q27
Cellular Component: extracellular space; extracellular region
Molecular Function: kinase inhibitor activity; cysteine protease inhibitor activity
Biological Process: pinocytosis; negative regulation of bone mineralization; ossification; positive regulation of phagocytosis; regulation of inflammatory response; acute-phase response; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; regulation of bone mineralization; skeletal development
Research Articles on AHSG
1. Fetuin-A and/or palmitate influence the expression of proinflammatory and angiogenic proteins only partially via TLR4 signalling
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
