Full Product Name
Goat anti-ALPL (aa42-53) Antibody
Product Synonym Names
ALPL; alkaline phosphatase, liver/bone/kidney; AP-TNAP; APTNAP; HOPS; TNAP; TNSALP; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; glycerophosphatase; liver/bone/kidney-type
Product Gene Name
anti-ALPL antibody
[Similar Products]
Antibody/Peptide Pairs
ALPL peptide (MBS428842) is used for blocking the activity of ALPL antibody (MBS423804)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P05186
Species Reactivity
Human
Expected from sequence similarity: Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen Sequence
Peptide with sequence C-ELQKLNTNVAKN, from the internal region (near N terminus) of the protein sequence according to NP_000469.3.
Peptide Sequence + C
C-ELQKLNTNVAKN
Epitope
Internal Region (near N terminus)
Note
This antibody is expected to recognize isoform 1 (NP_000469.3) only. The immunizing peptide represents the N terminus of the mature protein.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-ALPL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ALPL antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ALPL antibody
Pep-ELISA: antibody detection limit dilution 1:64000.
WB: Approx 55kDa band observed in Human Kidney, Lung and Adrenal Gland lysates. Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry (IHC) of anti-ALPL antibody
(0.1ug/ml) staining of Human Kidney (A), Lung (B) and Adrenal Gland (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for ALPL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000469.3
[Other Products]
NCBI GenBank Nucleotide #
NM_000478.5
[Other Products]
UniProt Primary Accession #
P05186
[Other Products]
UniProt Secondary Accession #
O75090; Q2TAI7; Q59EJ7; Q5BKZ5; Q5VTG5; Q6NZI8; Q8WU32; A1A4E7; B2RMP8; B7Z387; B7Z4Y6[Other Products]
UniProt Related Accession #
P05186[Other Products]
NCBI Official Full Name
alkaline phosphatase, tissue-nonspecific isozyme isoform 1 preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, liver/bone/kidney
NCBI Official Symbol
ALPL [Similar Products]
NCBI Official Synonym Symbols
HOPS; TNAP; TNALP; APTNAP; TNSALP; AP-TNAP
[Similar Products]
NCBI Protein Information
alkaline phosphatase, tissue-nonspecific isozyme
UniProt Protein Name
Alkaline phosphatase, tissue-nonspecific isozyme
UniProt Synonym Protein Names
Alkaline phosphatase liver/bone/kidney isozyme
Protein Family
Alkaline phosphatase
UniProt Gene Name
ALPL [Similar Products]
UniProt Synonym Gene Names
AP-TNAP; TNSALP [Similar Products]
NCBI Summary for ALPL
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]
UniProt Comments for ALPL
This isozyme may play a role in skeletal mineralization.
Research Articles on ALPL
1. Mutations in ALPL which reduce alkaline phosphatase activity are responsible for Hypophosphatasia , a rare disorder characterized by defective bone and teeth mineralization and early tooth loss.
Precautions
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Disclaimer
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