EG-VEGF, ELISA Kit

Human EG-VEGF

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (110993788..110999976). Location: 1p21

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

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Small volumes of EG-VEGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS355293 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EG-VEGF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EG-VEGF. The ELISA analytical biochemical technique of the MBS355293 kit is based on EG-VEGF antibody-EG-VEGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EG-VEGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EG-VEGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-EG-VEGF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-EG-VEGF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EG-VEGF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of EG-VEGF can be calculated.

Background/Introduction: Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) is a human protein encoded by the PROKR1 gene. It induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. EG-VEGF is mitogenic and chemoattractive and able to induce fenestration. Its expression is induced by hypoxia, and there is an HIF1 present on EG-VEGF. It is also able to induce angiogenesis and ovarian cyst formation.

Quantitative sELISA (EIA)

For quantitative detection of EG-VEGF in human serum, plasma, or cell culture supernatants.

11,715 Da

prokineticin 1

prokineticin-1; EG-VEGF; mambakine; black mamba toxin-related protein; endocrine-gland-derived vascular endothelial growth factor

Prokineticin-1

EG-VEGF  [Similar Products]

PROK1_HUMAN

The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]

Function: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. Ref.1 Ref.2 Ref.9 Ref.10 Ref.11

Subcellular location: Secreted.

Tissue specificity: Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta. Ref.2 Ref.9 Ref.11

Developmental stage: In ***** testis, is strongly expressed only in Leydig cells. In testicular tumors, expressed specifically in Leydig cell tumors (at protein level). Expressed from 14 weeks until birth in fetal testis.

Sequence similarities: Belongs to the AVIT (prokineticin) family.

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