Product Name
Vitellogenin (VTG), ELISA Kit
Popular Item
Full Product Name
Grouper Vitellogenin (VTG) ELISA Kit
Product Gene Name
VTG elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
Species Reactivity
Grouper
Specificity
This assay has high sensitivity and excellent specificity for detection of GrouperVTG. No significant cross-reactivity or interference between GrouperVTG and analogues was observed.
Samples
Serum, Plasma, Other
biological fluids
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of VTG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for VTG purchase
MBS280553 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vitellogenin (VTG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VTG. The ELISA analytical biochemical technique of the MBS280553 kit is based on VTG antibody-VTG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VTG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VTG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
VTG elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate VTG in samples. An antibody specific for VTG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVTG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VTG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VTG bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for VTG. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001011578.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001011578.1
[Other Products]
UniProt Related Accession #
Q868N5[Other Products]
Molecular Weight
201,048 Da
NCBI Official Full Name
vitellogenin
NCBI Official Synonym Full Names
vitellogenin
NCBI Official Symbol
Vg [Similar Products]
NCBI Official Synonym Symbols
GB13999
[Similar Products]
NCBI Protein Information
vitellogenin
UniProt Protein Name
Vitellogenin
Protein Family
Vitellogenin
UniProt Gene Name
Vg [Similar Products]
UniProt Entry Name
VIT_APIME
UniProt Comments for VTG
Precursor of the egg-yolk proteins that are sources of nutrients during embryonic development (). Involved in the differentiation of honeybee larvae into queens.
Research Articles on VTG
1. A tight coregulatory relationship exists between Vg and juvenile hormone (JH) in the regulation of honeybee behavioral maturation that is manifest at the genomic level; acting through common pathways, they regulate brain gene expression and behavior.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
