Full Product Name
anti-human CD7-FITC
Product Synonym Names
Monoclonal anti-human CD7/FITC
Product Gene Name
anti-CD7 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Performance
Five x 105 ficoll prepared peripheral blood mononuclear cells (PBMC) were washed and incubated 45 minutes on ice with 80 ul of anti-CD7/FITC at a 1:50 dilution(10ug/ml). Cells were washed three times, fixed and analyzed by FACS. A 67% sub population of the cells stained positive with a mean shift of 0.95 log10 fluorescent units when compared to a Mouse IgG2b/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD7 antibody.
Production
Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein/Antibody molar ratio of 4.6.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Human HSB2-T cells
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Other Notes
Small volumes of anti-CD7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD7 antibody
Information: Human CD7 is one of the earliest T lineage markers and is found on most thymocytes and peripheral blood T cells. The function of CD7 is not yet fully known, although cross-linking of CD7 with antibodies induces a T cell transmembrane calcium flux and CD7 expression is induced by ionomycin. Antibody 3Ale recognizes a CD7 molecule of 40 kd. Antibody 3Ale induces T cell transmembrane calcium flux.
Applications Tested/Suitable for anti-CD7 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD7 antibody
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test
Testing Data of anti-CD7 antibody
NCBI/Uniprot data below describe general gene information for CD7. It may not necessarily be applicable to this product.
NCBI Accession #
AAA51953.1
[Other Products]
UniProt Related Accession #
P09564[Other Products]
Molecular Weight
25,409 Da
NCBI Official Full Name
CD7 antigen protein
NCBI Official Synonym Full Names
CD7 molecule
NCBI Official Symbol
CD7 [Similar Products]
NCBI Official Synonym Symbols
GP40; TP41; Tp40; LEU-9
[Similar Products]
NCBI Protein Information
T-cell antigen CD7; p41 protein; CD7 antigen (p41); T-cell leukemia antigen; T-cell surface antigen Leu-9
UniProt Protein Name
T-cell antigen CD7
UniProt Synonym Protein Names
GP40; T-cell leukemia antigen; T-cell surface antigen Leu-9; TP41; CD_antigen: CD7
Protein Family
CD70 antigen
UniProt Gene Name
CD7 [Similar Products]
UniProt Entry Name
CD7_HUMAN
NCBI Summary for CD7
This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq, Jul 2008]
UniProt Comments for CD7
CD7: Not yet known.
Protein type: Membrane protein, integral; Immunoglobulin superfamily
Chromosomal Location of Human Ortholog: 17q25.2-q25.3
Cellular Component: membrane; plasma membrane; integral to membrane
Molecular Function: receptor activity
Biological Process: T cell activation; homeostasis of number of cells within a tissue; immune response; transmembrane receptor protein tyrosine kinase signaling pathway
Product References and Citations for anti-CD7 antibody
R.E. Ware, et al, (1989) J Immunol 143: 3632-3640. R.E. Ware, et al, (1991) J Immunol 147: 2787-2794.
Research Articles on CD7
1. CD7 is present on monocytes and tumor macrophages and its ligand, SECTM1, is frequently expressed in corresponding melanoma tissues
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
