Full Product Name
DKC1 siRNA (Rat)
Product Synonym Names
NAP57; H/ACA ribonucleoprotein complex subunit 4; Dyskerin; Nopp140-associated protein of 57 kDa; Nucleolar protein NAP57; Nucleolar protein family A member 4; snoRNP protein DKC1
Product Gene Name
DKC1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P40615
Specificity
DKC1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DKC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DKC1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DKC1 sirna
siRNA to inhibit DKC1 expression using RNA interference
Applications Tested/Suitable for DKC1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DKC1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_596910.1
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NCBI GenBank Nucleotide #
NM_133419.1
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UniProt Primary Accession #
P40615
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UniProt Secondary Accession #
Q499M9[Other Products]
UniProt Related Accession #
P40615[Other Products]
Molecular Weight
56,615 Da
NCBI Official Full Name
H/ACA ribonucleoprotein complex subunit 4
NCBI Official Synonym Full Names
dyskeratosis congenita 1, dyskerin
NCBI Official Symbol
Dkc1 [Similar Products]
NCBI Official Synonym Symbols
Nap57
[Similar Products]
NCBI Protein Information
H/ACA ribonucleoprotein complex subunit 4
UniProt Protein Name
H/ACA ribonucleoprotein complex subunit 4
UniProt Synonym Protein Names
Dyskerin; Nopp140-associated protein of 57 kDa; Nucleolar protein NAP57; Nucleolar protein family A member 4; snoRNP protein DKC1
UniProt Gene Name
Dkc1 [Similar Products]
UniProt Synonym Gene Names
Nap57 [Similar Products]
UniProt Entry Name
DKC1_RAT
NCBI Summary for DKC1
nucleolar protein expressed in liver; colocalized with Nopp140 protein; may be involved with rRNA processing [RGD, Feb 2006]
UniProt Comments for DKC1
dyskerin: Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Defects in DKC1 are a cause of dyskeratosis congenita X- linked recessive (XDKC). XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy. Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS). HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. Belongs to the pseudouridine synthase TruB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleolus; RNA-binding; Lyase; RNA processing; Isomerase; EC 5.4.99.-
Cellular Component: nucleoplasm; Cajal body; small nucleolar ribonucleoprotein complex; telomerase holoenzyme complex; nucleolus; nucleus
Molecular Function: telomerase activity; protein binding; pseudouridine synthase activity; RNA binding
Biological Process: RNA processing; cell proliferation; box H/ACA snoRNA metabolic process; pseudouridine synthesis; rRNA pseudouridine synthesis; RNA-dependent DNA replication; positive regulation of telomerase activity; rRNA processing; positive regulation of telomere maintenance via telomerase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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