Arachidonate 12-lipoxygenase, 12R-type (ALOX12B), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ALOX12B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7228029 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Arachidonate 12-lipoxygenase, 12R-type (ALOX12B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALOX12B. The ELISA analytical biochemical technique of the MBS7228029 kit is based on ALOX12B antibody-ALOX12B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALOX12B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALOX12B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

80,356 Da

arachidonate 12-lipoxygenase, 12R type

arachidonate 12-lipoxygenase, 12R-type; 12R-lipoxygenase; epidermis-type lipoxygenase 12

Arachidonate 12-lipoxygenase, 12R-type

12R-LOX; 12R-lipoxygenase  [Similar Products]

LX12B_HUMAN

This gene encodes an enzyme involved in the converstion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma. [provided by RefSeq, Jul 2008]

ALOX12B: Converts arachidonic acid to 12R- hydroperoxyeicosatetraenoic acid (12R-HPETE). Defects in ALOX12B are a cause of non-bullous congenital ichthyosiform erythroderma (NCIE). NCIE is a non- bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. Most affected individuals are born with a tight, shiny, translucent covering called collodion membrane. The collodion membrane subsequently evolves into generalized scaling and intense redness of the skin. Clinical features are milder than in lamellar ichthyoses and demonstrate a greater variability in the intensity of erythema, size and type of scales. In contrast to lamellar ichthyoses, scales are usually white, fine and powdery, and palms and soles are severely affected. Patients suffer from palmoplantar keratoderma, often with painful fissures, digital contractures, and loss of pulp volume. Belongs to the lipoxygenase family.

Protein type: EC 1.13.11.-; Oxidoreductase; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytosol

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; arachidonate 12-lipoxygenase activity; iron ion binding

Biological Process: sphingolipid metabolic process; positive regulation of MAPKKK cascade; lipoxygenase pathway; protein amino acid lipidation; arachidonic acid metabolic process; linoleic acid metabolic process; ceramide biosynthetic process; hepoxilin biosynthetic process

Disease: Ichthyosis, Congenital, Autosomal Recessive 2

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